Isolation and identification of phagocytic cells.

Details

• Personal Author:
  Castranova, Vincent ; Dyke K ; Scott MR
• Description:
  Methods for purifying phagocytic cells from various tissues were reviewed. The isolation and identification of pulmonary macrophages were discussed and methods for isolating alveolar macrophages in laboratory animals, for obtaining in-situ pulmonary macrophages from experimental animals, for identifying alveolar macrophages, for isolating and identifying blood phagocytes, for isolating monocytes and neutrophilic granulocytes from human blood, for purifying monocytes and polymorphonuclear leukocytes, for purifying blood granulocytes by centrifugal elutriation, for isolating and purifying peritoneal phagocytes, and for isolating and purifying hepatic Kupffer cells from mice were described. The heterogeneity of cellular preparations was discussed. It was noted that in-situ pulmonary macrophages exhibit greater uptake of particles and greater secretion of superoxide anion than free alveolar macrophages. Methods for separating subpopulations of alveolar macrophages by density and for separating dog alveolar macrophages by centrifugal elutriation were summarized. The heterogeneity of peritoneal macrophages and blood neutrophils were also considered. [Description provided by NIOSH]
• Subjects:
  Biological Products Blood Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Laboratory-animals; Lung-cells; Blood-cells; Humans; Phagocytic-activity; Laboratory-techniques; Laboratory-testing; Liver-cells; Biological-material; NIOSH-Author
• ISBN:
  9780849343322
• Publisher:
  CRC Press
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Florida ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  25-38
• NIOSHTIC Number:
  nn:00176483
• Citation:
  Cellular chemiluminescence. Van Dyke K, Castranova V, eds. Boca Raton, FL: CRC Press, 1987 Jul; :25-38
• Editor(s):
  Van Dyke K; Castranova V
• Federal Fiscal Year:
  1987
• Peer Reviewed:
  False
• Source Full Name:
  Cellular chemiluminescence
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242425/cdc_242425_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]