Phosphoinositide metabolism in peripheral axons.

Details

• Personal Author:
  Gould RM ; Spencer RJ
• Description:
  Uptake and incorporation of myo-inositol (87898) and phosphate (14265442) into squid and mouse nerve fibers were examined. Tritiated myo-inositol and choline (62497) were used as precursors of the polar headgroups of phosphatidylinositol and phosphatidylcholine. Incubation of squid axoplasm with these precursors and with cytidine-diphosphocholine (987780) yielded more incorporation of myo-inositol than choline into lipid. Autoradiography of mouse sciatic nerve demonstrated that inositol was selectively incorporated into lipid in the axoplasm. The distribution of silver grains over the Schwann cell cytoplasm suggested that both choline and myo-inositol lipid syntheses initially occur in this cell. Some of the lipid formed in Schwann cell cytoplasm was transferred into myelin when incorporation periods were lengthened and some of the phosphatidylinositol formed in the axoplasm was transferred to a region overlaying the axolemma. Inorganic phosphate labeled with 33-P was used to characterize further the axon associated lipid metabolism in mouse myelinated fibers. This precursor was incorporated largely into phosphatidylinositol-bisphosphate and protein. Quantitative examination of electron microscope autoradiographs of cross sectioned fibers revealed sites of incorporation localized to junctions where Schwann cell and axon plasma membranes were located. The authors suggest that phosphatidylinositol and protein are phosphorylated at sites in these membranes. [Description provided by NIOSH]
• Subjects:
  Cytology Energy Metabolism Laboratories Lipids Nervous System Occupational Health Safety
• Keywords:
  NIOSH-Grant; Neurotoxic-effects; Phospholipids; Neurophysiology; Peripheral-nervous-system; Cellular-function; Cytological-analysis; Analytical-methods; Metabolic-effects; Laboratory-animals; Research-laboratories
• ISBN:
  9780127417509
• Publisher:
  Academic Press
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Book
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:00091810
• Citation:
  Cyclitols and phosphoinositides. Wells WW, Eisenberg F, eds. New York: Academic Press, Inc., 1978:535-547
• Contact Point Address:
  Pathology Albert Einstein Coll of Med 1300 Morris Park Avenue Bronx, N Y 10461
• CAS Registry Number:
  Choline (CAS RN 62-49-7) ; Cytidine diphosphate choline (CAS RN 987-78-0) ; myo-Inositol (CAS RN 87-89-8) ; Phosphate (CAS RN 14265-44-2)
• Editor(s):
  Wells WW; Eisenberg F
• Federal Fiscal Year:
  1978
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  False
• Start Date:
  19751101
• Source Full Name:
  Cyclitols and phosphoinositides
• End Date:
  19850331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/240922/cdc_240922_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]