Candidate Cell Substrates, Vaccine Production, and Transmissible Spongiform Encephalopathies

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Piccardo, Pedro ; Cervenakova, Larisa ; Vasilyeva, Irina ; Yakovleva, Oksana ; Bacik, Igor ; Cervenak, Juraj ; McKenzie, Carroll ; Kurillova, Lubica ; Gregori, Luisa ; Pomeroy, Kitty ; Asher, David M.
• Description:
  Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.
• Subjects:
  Animals Biological Assay Bovine Spongiform Encephalopathy BSE Cattle Cell Culture Techniques Cell Line Cercopithecus Aethiops CHO Cells CJD Communicable Diseases, Emerging Creutzfeldt-Jakob Disease Creutzfeldt-Jakob Syndrome Cricetinae Cricetulus Disease Models, Animal Dogs Drug Contamination Encephalopathy, Bovine Spongiform HEK293 Cells Humans Mice Mice, Transgenic Prion Prion Diseases Prion Protein Prions Research Saimiri Scrapie Transmissible Spongiform Encepthalopathy TSE Vaccines Vero Cells

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• Source:
  Emerg Infect Dis. 17(12):2262-2269.
• Document Type:
  Journal Article
• Funding:
  Y1-AI-4893-02/AI/NIAID NIH HHS/United States
• Volume:
  17
• Issue:
  12
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:6c41fc7262f299a7f18acbadd21dafe8fd5a99f28422aea21c0ff682eeee837c
• Download URL:
  https://stacks.cdc.gov/view/cdc/23779/cdc_23779_DS1.pdf
• File Type:
  [PDF - 370.73 KB ]