Candidate Cell Substrates, Vaccine Production, and Transmissible Spongiform Encephalopathies
Published Date:Dec 2011
Source:Emerg Infect Dis. 17(12):2262-2269.
Bovine Spongiform Encephalopathy
Cell Culture Techniques
Communicable Diseases, Emerging
Disease Models, Animal
Encephalopathy, Bovine Spongiform
Transmissible Spongiform Encepthalopathy
Funding:Y1-AI-4893-02/AI/NIAID NIH HHS/United States
Description:Transmissible spongiform encephalopathy (TSE) agents have contaminated human tissue-derived medical products, human blood components, and animal vaccines. The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). Exposed cultures were tested for TSE-associated prion protein (PrP(TSE)) and TSE infectivity by assay in rodents and nonhuman primates. No PrP(TSE) or infectivity has been detected in any exposed cell line under study so far. Animals inoculated with BSE brain homogenate developed typical spongiform encephalopathy. In contrast, animals inoculated with cells exposed to the BSE agent remained asymptomatic. All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent.
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