Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid1801.110589
Personal Author:
Notari, Silvio; Qing, Liuting; Pocchiari, Maurizio; Dagdanova, Ayuna; Hatcher, Kristin; ... More +
Notari, Silvio; Qing, Liuting; Pocchiari, Maurizio; Dagdanova, Ayuna; Hatcher, Kristin; Dogterom, Arend; Groisman, Jose F.; Lumholtz, Ib Bo; Puopolo, Maria; Lasmezas, Corinne; Chen, Shu G.; Kong, Qingzhong; Gambetti, Pierluigi; Less -
Description:
Prion diseases are neurodegenerative conditions associated with a misfolded and infectious protein, scrapie prion protein (PrP(Sc)). PrP(Sc) propagate prion diseases within and between species and thus pose risks to public health. Prion infectivity or PrP(Sc) presence has been demonstrated in urine of experimentally infected animals, but there are no recent studies of urine from patients with Creutzfeldt-Jakob disease (CJD). We performed bioassays in transgenic mice expressing human PrP to assess prion infectivity in urine from patients affected by a common subtype of sporadic CJD, sCJDMM1. We tested raw urine and 100-fold concentrated and dialyzed urine and assessed the sensitivity of the bioassay along with the effect of concentration and dialysis on prion infectivity. Intracerebral inoculation of transgenic mice with urine from 3 sCJDMM1 patients failed to demonstrate prion disease transmission, indicating that prion infectivity in urine from sCJDMM1 patients is either not present or is <0.38 infectious units/mL.
Subject:
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Animals
Biological Assay
Brain
Creutzfeld-Jakob Disease
Creutzfeldt-Jakob Syndrome
Humans
Infectivity
Mice
Mice, Transgenic
Neurodegenerative Diseases
Prions
Prions And Related Diseases
Research
Sensitivity And Specificity
Transmissible Spongiform Encephalopathy
TSE
Urine

Source:
Emerg Infect Dis. 18(1):21-28.;
Document Type:
Journal Article;
Funding:
AG014359/AG/NIA NIH HHS/United States; NS052319/NS/NINDS NIH HHS/United States; UR8/CCU515004/PHS HHS/United States;
AG014359/AG/NIA NIH HHS/United States; NS052319/NS/NINDS NIH HHS/United States; UR8/CCU515004/PHS HHS/United States; Less -
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:dc6dc9978f2af7c9bca12c49e90981cbe1ad0dc8e74aa510062c68a3cc300c21
File Type:

Supporting Files

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