Agricultural Dust Derived Bacterial Extracellular Vesicle Mediated Inflammation Is Attenuated by DHA

Details

• Personal Author:
  Heires AJ ; Nordgren TM ; Romberger DJ ; Samuelson D ; Villageliu D
• Description:
  Dietary long-chain omega-3 polyunsaturated fatty acids (n-3 PUFA) and their pro-resolving metabolites are protective against atherosclerotic disease, and ameliorate systemic inflammatory conditions including lupus erythematosus, psoriasis, and bronchial asthma. Organic bioaerosol inhalation is a common and injurious hazard associated with agricultural occupations such as work in swine concentrated animal feeding operations (CAFOs) and is known to increase the risk for developing respiratory conditions such as asthma and COPD. Nearly all cells secrete membrane-bound vesicles (extracellular vesicles, EVs) that have the capacity to transmit protein, nucleic acid, and lipid signaling mediators between cells. Using a polymer-based isolation technique (ExoQuick, PEG) followed by ultracentrifugation, EVs were isolated from CAFO dust extracts, and were quantified and partially characterized. Here, we investigated the role of the n-3 PUFA docosahexaenoic acid (DHA) as a component of n-6 to n-3 PUFA mixtures used to recapitulate physiologically relevant dietary ratios in the resolution of inflammatory injury caused by exposure to EVs carried by agricultural organic dust in vitro. Primary human bronchial epithelial cells, fibroblasts and monocyte-derived macrophages were exposed to EVs isolated from swine CAFO dust. Cells were treated with mixtures of n-6 and n-3 PUFA during recovery from the EV-induced injury. CAFO dust extract (DE) was found to contain EVs that contributed significantly to the overall consequences of exposure to complete DE. DHA-rich PUFA ratios inhibited DE-derived EV-induced proinflammatory cytokine release dose-dependently. DHA-rich PUFA ratios also reversed the damaging effects of EVs on recellularization of lung matrix scaffolds, accelerated wound healing, and stimulated the release of pro-resolution mediators. These results underscore the importance of n-3 PUFA as anti-inflammatory compounds during recovery from EV-laden environmental dust exposure in the context of cellular responses in vitro, warranting future translational studies. [Description provided by NIOSH]
• Subjects:
  Agriculture Asthma Diet Dust Energy Metabolism Environmental Exposure Lipids Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Agricultural Bioaerosols Bronchial Asthma Cellular Function Chronic Obstructive Pulmonary Disease COPD Dusts Environmental Exposure Fatty Acids Metabolites Nucleic Acids Organic Compounds Proteins Respiratory System Disorders

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• ISSN:
  2045-2322
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Nebraska ; OSHA Region 7 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• NIOSHTIC Number:
  nn:20066955
• Citation:
  Sci Rep 2023 Feb; 13:2767
• Contact Point Address:
  Debra J. Romberger, Department of Internal Medicine, Pulmonary, Critical Care & Sleep division, University of Nebraska Medical Center, Omaha, NE 68198
• Email:
  dromberg@unmc.edu
• Federal Fiscal Year:
  2023
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Scientific Reports
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f7b749a8d881f089aac7f60c83a647f4d5a8ee1bd5a8705dcce9493d2ff5395b02e6a3ced71d20a49be97b99daf79bc05cd62c4e8e0621ef9a4ee95235ed5281
• Download URL:
  https://stacks.cdc.gov/view/cdc/231225/cdc_231225_DS1.pdf
• File Type:
  [PDF - 3.21 MB ]