Β-Defensin-1 Regulates Influenza Virus Infection in Human Bronchial Epithelial Cells Through the STAT3 Signaling Pathway (Dataset)

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  Understanding the host response to influenza A virus (IAV) infection is vital for developing intervention strategies. The primary barriers for invading respiratory pathogens are the airway epithelial cells of the respiratory tract and antimicrobial peptides produced by these cells. The antimicrobial peptide, ß-defensin-1, has antiviral activity against both enveloped and non-enveloped viruses. Significant downregulation of ß-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. HBEpCs overexpressing DEFB1 caused a significant reduction in IAV, that was confirmed by IAV matrix gene analysis and confocal microscopy. DEFB1expression after transfection with hsa-miR-186-5p and hsa-miR-340-5p provided evidence that DEFB1 expression could be modulated by these two miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. Overexpression of DEFB1 in IAV infected HBEpCs led to increased NF-kB expression. In a Polymerase Chain Reaction (PCR) array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of STAT3. In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered by cells overexpressing DEFB1, suggesting that this may be one of the pathways by which defensin regulates IAV replication in HBEpCs. In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that ß-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. [Description provided by NIOSH]
• Subjects:
  Communicable Diseases Immune System Influenza, Human Occupational Health Respiratory System Respiratory Tract Diseases Safety Viral Diseases
• Keywords:
  Cell Signaling Gene Expression Immune Reaction Influenza Pathogens Proteins Respiratory Infections Viral Diseases
• DOI:
  https://doi.org/10.26616/NIOSHRD-1053-2023-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20066862
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1053-2023-0, 2023 Jan; :dataset
• Contact Point Address:
  Allergy and Clinical Immunology Branch (ACIB), Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV. Phone: 304.285.6024
• Federal Fiscal Year:
  2023
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  False
• Source Full Name:
  ß-defensin-1 regulates influenza virus infection in human bronchial epithelial cells through the STAT3 signaling pathway
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0f20d67148d97ee1a48282baa90bb30ee166b9546f678764e7f2b35843ca10255a328f9efd28cc6cc80d5f8dac22701c7f325ff9587cd6be76bb0074c86bae5a
• Download URL:
  https://stacks.cdc.gov/view/cdc/231181/cdc_231181_DS1.pdf
• File Type:
  [PDF - 200.96 KB ]