Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0036023
Personal Author:
Wong, Katy A. ; Wilson, Jessica ; Russo, Angela ; Wang, Li ; Okur, Mustafa Nazir ; ... More +
Wong, Katy A. ; Wilson, Jessica ; Russo, Angela ; Wang, Li ; Okur, Mustafa Nazir ; Wang, Xuerong ; Martin, Negin P. ; Scappini, Erica ; Carnegie, Graeme K. ; O'Bryan, John P. Less -
Description:
Members of the intersectin (ITSN) family of scaffold proteins consist of multiple modular domains, each with distinct ligand preferences. Although ITSNs were initially implicated in the regulation of endocytosis, subsequent studies have revealed a more complex role for these scaffold proteins in regulation of additional biochemical pathways. In this study, we performed a high throughput yeast two-hybrid screen to identify additional pathways regulated by these scaffolds. Although several known ITSN binding partners were identified, we isolated more than 100 new targets for the two mammalian ITSN proteins, ITSN1 and ITSN2. We present the characterization of several of these new targets which implicate ITSNs in the regulation of the Rab and Arf GTPase pathways as well as regulation of the disrupted in schizophrenia 1 (DISC1) interactome. In addition, we demonstrate that ITSN proteins form homomeric and heteromeric complexes with each other revealing an added level of complexity in the function of these evolutionarily conserved scaffolds.
Subject:
[+]
Adaptor Proteins, Vesicular Transport
Animals
Biology
Cercopithecus Aethiops
COS Cells
Endocytosis
GTP Phosphohydrolases
HEK293 Cells
High-Throughput Screening Assays
Humans
Multigene Family
Nervous System Diseases
Phosphatidylinositols
Protein Binding
Protein Interaction Maps
Protein Multimerization
Receptor Protein-Tyrosine Kinases
Research Article
Signal Transduction
Two-Hybrid System Techniques

Source:
PLoS One. 2012; 7(4).
Pubmed ID:
22558309
Pubmed Central ID:
PMC3338775
Document Type:
Journal Article
Funding:
HL090651/HL/NHLBI NIH HHS/United States ; PR080428/PR/OCPHP CDC HHS/United States
HL090651/HL/NHLBI NIH HHS/United States ; PR080428/PR/OCPHP CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:4700c73552fd12bee0f9de5c907de7855846421cb6189e808b8e137eb4629303
File Type:

Supporting Files

• 	pone.0036023.g004.jpg	jpeg
  	pone.0036023.g005.gif	gif
  	pone.0036023.g005.jpg	jpeg
  	pone.0036023.nxml	txt
  	pone.0036023.s001.pdf	pdf
  	pone.0036023.s002.pdf	pdf
  	pone.0036023.t001.gif	gif
  	pone.0036023.t001.jpg	jpeg
  	license.txt	txt
  	pone.0036023.g001.gif	gif
  	pone.0036023.g001.jpg	jpeg
  	pone.0036023.g002.gif	gif
  	pone.0036023.g002.jpg	jpeg
  	pone.0036023.g003.gif	gif
  	pone.0036023.g003.jpg	jpeg
  	pone.0036023.g004.gif	gif

More +