Details:

Alternative Title:
Nat Commun
Personal Author:
Önder, Özlem ; Shao, Wenguang ; Kemps, Brian D. ; Lam, Henry ; Brisson, Dustin
Önder, Özlem ; Shao, Wenguang ; Kemps, Brian D. ; Lam, Henry ; Brisson, Dustin Less -
Description:
Rapid and reliable identification of the vertebrate species on which a disease vector previously parasitized is imperative to study ecological factors that affect pathogen distribution and can aid the development of public health programs. Here we describe a proteome profiling technique designed to identify the source of blood meals of haematophagous arthropods. This method employs direct spectral matching and thus does not require a priori knowledge of any genetic or protein sequence information. Using this technology, we detect remnants of blood in blacklegged ticks (Ixodes scapularis) and correctly determine the vertebrate species from which the blood was derived, even 6 months after the tick had fed. This biological fingerprinting methodology is sensitive, fast, cost-effective and can potentially be adapted for other biological and medical applications when existing genome-based methods are impractical or ineffective.
Subject:
[+]
Algorithms
Animals
Article
Cluster Analysis
Conserved Sequence
Evolution, Molecular
Feeding Behavior
Larva
Mice
Molting
Peptide Library
Proteome
Proteomics
Species Specificity
Tandem Mass Spectrometry
Ticks
Vertebrates

Source:
Nat Commun. 4:1746
Pubmed ID:
23612287
Pubmed Central ID:
PMC3635114
Document Type:
Journal Article
Funding:
AI076342/AI/NIAID NIH HHS/United States ; Intramural NIH HHS/United States ; U01CK000170/CK/NCEZID CDC HHS/United States ; R01 AI076342/AI/NIAID NIH HHS/United States ; R01 AI097137/AI/NIAID NIH HHS/United States
AI076342/AI/NIAID NIH HHS/United States ; Intramural NIH HHS/United States ; U01CK000170/CK/NCEZID CDC HHS/United States ; R01 AI076342/AI/NIAID NIH HHS/United States ; R01 AI097137/AI/NIAID NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9ea65f15655e90b28f598e31b3f3613cdc4feee1fb3284f8143d8d5d059c73cd
File Type:

Supporting Files

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