Identifying sources of tick blood meals using unidentified tandem mass spectral libraries

Details

• Alternative Title:
  Nat Commun
• Personal Author:
  Önder, Özlem ; Shao, Wenguang ; Kemps, Brian D. ; Lam, Henry ; Brisson, Dustin
• Description:
  Rapid and reliable identification of the vertebrate species on which a disease vector previously parasitized is imperative to study ecological factors that affect pathogen distribution and can aid the development of public health programs. Here we describe a proteome profiling technique designed to identify the source of blood meals of haematophagous arthropods. This method employs direct spectral matching and thus does not require a priori knowledge of any genetic or protein sequence information. Using this technology, we detect remnants of blood in blacklegged ticks (Ixodes scapularis) and correctly determine the vertebrate species from which the blood was derived, even 6 months after the tick had fed. This biological fingerprinting methodology is sensitive, fast, cost-effective and can potentially be adapted for other biological and medical applications when existing genome-based methods are impractical or ineffective.
• Subjects:
  Algorithms Animals Article Cluster Analysis Conserved Sequence Evolution, Molecular Feeding Behavior Larva Mice Molting Peptide Library Proteome Proteomics Species Specificity Tandem Mass Spectrometry Ticks Vertebrates

  More +
• Source:
  Nat Commun. 4:1746
• Pubmed ID:
  23612287
• Pubmed Central ID:
  PMC3635114
• Document Type:
  Journal Article
• Funding:
  AI076342/AI/NIAID NIH HHS/United States ; Intramural NIH HHS/United States ; U01CK000170/CK/NCEZID CDC HHS/United States ; R01 AI076342/AI/NIAID NIH HHS/United States ; R01 AI097137/AI/NIAID NIH HHS/United States
• Volume:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:9ea65f15655e90b28f598e31b3f3613cdc4feee1fb3284f8143d8d5d059c73cd
• Download URL:
  https://stacks.cdc.gov/view/cdc/22562/cdc_22562_DS1.pdf
• File Type:
  [PDF - 1.37 MB ]