Tracking the sources of blood meals of parasitic arthropods using shotgun proteomics and unidentified tandem mass spectral libraries

Details

• Alternative Title:
  Nat Protoc
• Personal Author:
  Önder, Özlem ; Shao, Wenguang ; Lam, Henry ; Brisson, Dustin
• Description:
  Identifying the species on which hematophagous arthropods feed is crucial for studying the factors that affect pathogen distributions and that can aid public health. Here we describe a protocol to identify the species a parasitic arthropod has previously fed upon by identifying the source of the remnants of a previous blood meal via shotgun proteomics and spectral matching. The protocol is a nontargeted approach that uses the entire detected blood proteome for source identification; it does not require a priori knowledge of genome or protein sequences. Instead, reference spectral libraries are compiled from the blood of multiple host species by using SpectraST, which takes ∼4 d; the identification of the species from which a previous blood meal of a hematophagous arthropod was taken is achieved with spectral matching against the reference spectral libraries, which takes approximately another 4 d. This method is robust against random degradation of the blood meal and can identify unknown blood remnants months after the feeding event.
• Keywords:
  Animals Arthropods Blood Ixodes Proteomics Tandem Mass Spectrometry Vertebrates
• Source:
  Nat Protoc. 2014; 9(4):842-850
• Pubmed ID:
  24625782
• Pubmed Central ID:
  PMC4179104
• Document Type:
  Journal Article
• Funding:
  R01 AI076342/AI/NIAID NIH HHSUnited States/ ; R01 AI097137/AI/NIAID NIH HHSUnited States/ ; U01CK000170/CK/NCEZID CDC HHSUnited States/ ; AI076342/AI/NIAID NIH HHSUnited States/
• Volume:
  9
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7e13b27a4e90d7b545f087d754f13fa6c1d338097ed82435eb1dd7b18ef18043
• Download URL:
  https://stacks.cdc.gov/view/cdc/30130/cdc_30130_DS1.pdf
• File Type:
  [PDF - 1.57 MB ]