Approaches for Characterizing and Tracking Hospital-Associated Multidrug-Resistant Bacteria

Details

• Personal Author:
  Blake KS ; Choi J ; Dantas G
• Description:
  Hospital-associated infections are a major concern for global public health. Infections with antibiotic-resistant pathogens can cause empiric treatment failure, and for infections with multidrug-resistant bacteria which can overcome antibiotics of "last resort" there exists no alternative treatments. Despite extensive sanitization protocols, the hospital environment is a potent reservoir and vector of antibiotic-resistant organisms. Pathogens can persist on hospital surfaces and plumbing for months to years, acquire new antibiotic resistance genes by horizontal gene transfer, and initiate outbreaks of hospital-associated infections by spreading to patients via healthcare workers and visitors. Advancements in next-generation sequencing of bacterial genomes and metagenomes have expanded our ability to (1) identify species and track distinct strains, (2) comprehensively profile antibiotic resistance genes, and (3) resolve the mobile elements that facilitate intra- and intercellular gene transfer. This information can, in turn, be used to characterize the population dynamics of hospital-associated microbiota, track outbreaks to their environmental reservoirs, and inform future interventions. This review provides a detailed overview of the approaches and bioinformatic tools available to study isolates and metagenomes of hospital-associated bacteria, and their multi-layered networks of transmission. [Description provided by NIOSH]
• Subjects:
  Anti-Bacterial Agents Communicable Diseases Genetics Health Care Facilities Workforce And Services Occupational Health Safety
• Keywords:
  Antibiotics Author Keywords: Antibiotic Resistance Bacteria Genes Hospital ICU Infection Control Infectious Diseases Medical Facilities Microbiome Mobilome Next-generation Sequencing Pathogens Resistome Sanitation

  More +
• ISSN:
  1420-682X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Missouri ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• Issue:
  6
• NIOSHTIC Number:
  nn:20062059
• Citation:
  Cell Mol Life Sci 2021 Mar; 78(6):2585-2606
• Contact Point Address:
  Kevin S. Blake, The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO 63110
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Washington University, Saint Louis
• Peer Reviewed:
  True
• Start Date:
  20180901
• Source Full Name:
  Cellular and Molecular Life Sciences
• End Date:
  20220831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bbd7e0f056b8b26808b9a7fb6b8050119d553f8138999192f0364249ebb4ae087ff30683eda426f74563aaf388c96764ce6f9e7be44297b48c05dd318df9be56
• Download URL:
  https://stacks.cdc.gov/view/cdc/225378/cdc_225378_DS1.pdf
• File Type:
  [PDF - 777.18 KB ]