Disparate Antibiotic Resistance Gene Quantities Revealed Across 4 Major Cities in California: A Survey in Drinking Water, Air, and Soil at 24 Public Parks

Details

• Personal Author:
  Echeverria-Palencia CM ; Ericksen CA ; Ficara E ; Gamboa D ; Haro H ; Hernandez-Cira M ; Jay JA ; Li R ; Melendez I ; Paulson SE ; Sanchez MG ; Senthilkumar N ; Sun F ; Thulsiraj V ; Tran N ; Walpert D ; Yuan T ; Zhu Y
• Description:
  Widespread prevalence of multidrug and pandrug-resistant bacteria has prompted substantial concern over the global dissemination of antibiotic resistance genes (ARGs). Environmental compartments can behave as genetic reservoirs and hotspots, wherein resistance genes can accumulate and be laterally transferred to clinically relevant pathogens. In this work, we explore the ARG copy quantities in three environmental media distributed across four cities in California and demonstrate that there exist city-to-city disparities in soil and drinking water ARGs. Statistically significant differences in ARGs were identified in soil, where differences in blaSHV gene copies were the most striking; the highest copy numbers were observed in Bakersfield (6.0 × 10-2 copies/16S-rRNA gene copies and 2.6 × 106 copies/g of soil), followed by San Diego (1.8 × 10-3 copies/16S-rRNA gene copies and 3.0 × 104 copies/g of soil), Fresno (1.8 × 10-5 copies/16S-rRNA gene copies and 8.5 × 102 copies/g of soil), and Los Angeles (5.8 × 10-6 copies/16S-rRNA gene copies and 5.6 × 102 copies/g of soil). In addition, ARG copy numbers in the air, water, and soil of each city are contextualized in relation to globally reported quantities and illustrate that individual genes are not necessarily predictors for the environmental resistome as a whole. [Description provided by NIOSH]
• Subjects:
  Anti-Bacterial Agents Genetics Occupational Health Safety
• Keywords:
  Antibiotics Bacteria Drinking Water Genes MDR MDROs Multidrug Resistant Multidrug Resistant Organisms Pathogens Soil Bacteria
• ISSN:
  2470-1343
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  2
• Issue:
  5
• NIOSHTIC Number:
  nn:20055730
• Citation:
  ACS Omega 2017 May; 2(5):2255-2263
• Contact Point Address:
  Jennifer A. Jay, Civil and Environmental Engineering Department, University of California at Los Angeles, 420 Westwood Plaza, Los Angeles, California 90095, United States
• Email:
  jjay@seas.ucla.edu
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  ACS Omega
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:920f5ae535670a31911495e918b8befec95e97d095d2dd4d31b16a4119af982abe93f22999f80bd1aa4a6dc1158833643db40531d2bcea5b65b4da50a7a16736
• Download URL:
  https://stacks.cdc.gov/view/cdc/213512/cdc_213512_DS1.pdf
• File Type:
  [PDF - 2.16 MB ]