Skin Tests in a Primate Model of Allergic Bronchopulmonary Aspergillosis

Details

• Personal Author:
  Fischer VW ; Hutcheson PS ; Slavin RG ; Tsai CC
• Description:
  We have previously reported producing a primate model of allergic bronchopulmonary aspergillosis in which monkey precipitating IgG and transfused human IgE antibodies against Aspergillus fumigatus (AF) combined with aerosolized AF to produce an inflammatory response in the lung. In this study we attempt to demonstrate similar changes in the skin. Immunized and unimmunized monkeys with and without IgG precipitating antibody to AF were injected at multiple sites intradermally with normal human serum or human serum rich in IgE against AF. 1 day later each site was injected with AF. Serial skin biopsies were taken at intervals of 15min and 1, 2, 6, 12, 24, and 48 h and light microscopic, immunofluorescent, and electron microscopic studies were performed. The most profound changes were associated with simian antibody (IgG) and human antibody (IgE) directed against AF and consisted of perivascular and interstitial neutrophilic infiltration at 2 h, eosinophils at 6 h, and mononuclar cells at 24 h. Immunofluorescent staining for fibrin-fibrinogen, IgM, and C3 was present and diapedesis of cells to extra-vascular dermal areas was evident. The skin does appear to mirror the inflammatory changes seen in the lung in this primate model of allergic bronchopulmonary aspergillosis and should afford a useful model for further studies. [Description provided by NIOSH]
• Subjects:
  Animals Antibodies Fungi Immune System Occupational Health Respiratory System Respiratory Tract Diseases Safety Skin
• Keywords:
  Animal Studies Aspergillosis Aspergillus Infection Immunology Respiratory Diseases Skin Tests
• ISSN:
  0020-5915
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Missouri ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  241-249
• Volume:
  65
• Issue:
  3
• NIOSHTIC Number:
  nn:20061218
• Citation:
  Int Arch Allergy Appl Immunol 1981 Jul; 65(3):241-249
• Contact Point Address:
  Dr. Raymond G. Slavin, Department of Internal Medicine, Division of Allergy and Immunology, Saint Louis University School of Medicine, Room 210, Doisy Hall, 1402 South Grand Boulevard, Saint Louis, MO 63104
• Federal Fiscal Year:
  1981
• Performing Organization:
  St. Louis University, Saint Louis, Missouri
• Peer Reviewed:
  True
• Start Date:
  19740401
• Source Full Name:
  International Archives of Allergy and Applied Immunology
• End Date:
  19800731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:88680417a525ad8c0d6291d854e419c8651756cf1e40a05b560aad079da163307f735bcce422b3ef8bd53fa3f6290964be85fb07d162d23d3674ea21e3aa746e
• Download URL:
  https://stacks.cdc.gov/view/cdc/224828/cdc_224828_DS1.pdf
• File Type:
  [PDF - 925.71 KB ]