Pineal Modulation of ACTH 1–17 Effect upon Murine Corticosterone Production

Details

• Personal Author:
  Brown G ; Cornelissen G ; Halberg E ; Halberg F ; Sánchez de la Peña S ; Sánchez E ; Scheving LE ; Ungar F ; Vecsei P ; Yunis EG
• Description:
  In tests of Corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37 degrees C with 95% O2 and 5% CO2, the media were stored at -20 degrees C until Corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal Corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward. [Description provided by NIOSH]
• Subjects:
  Animals Circadian Rhythm Occupational Health Safety
• Keywords:
  ACTH 1-17 Adrenal Adrenocortical Hormones Amplification Animal Studies Attenuation Author Keywords: Pineal Biological Rhythms Circadian Rhythms Corticosteroids Feed-sideward Hormone Activity Incubation

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• ISSN:
  0361-9230
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; Massachusetts ; Minnesota ; OSHA Region 1 ; OSHA Region 5 ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  117-125
• Volume:
  11
• Issue:
  1
• NIOSHTIC Number:
  nn:20060772
• Citation:
  Brain Res Bull 1983 Jul; 11(1):117-125
• Contact Point Address:
  Dr. Franz Halberg, Chronobiology Labs., University of Minnesota, Medcine and Pathology, 5-187 Lyon Labs, Minneapolis, 420 Washington Ave. S.E., Minneapolis, MN 55455
• Federal Fiscal Year:
  1983
• Performing Organization:
  University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
• Peer Reviewed:
  True
• Start Date:
  19791201
• Source Full Name:
  Brain Research Bulletin
• End Date:
  19880301
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4ad5e03cd51fe3a71f2546eef485ce8129582a36acdf76ea4962ede681de42d90b0ee8a06436ccf500c353c50dd21c81c41636e03af171fe820bf03a7cadbb43
• Download URL:
  https://stacks.cdc.gov/view/cdc/224593/cdc_224593_DS1.pdf
• File Type:
  [PDF - 662.42 KB ]