Circadian Pineal Modulation of Pituitary Effect on Murine Corticosterone In Vitro

Details

• Personal Author:
  Halberg F ; Haus E ; Lakatua D ; Sánchez de la Peña S ; Sánchez E ; Scheving LE ; Ungar F ; Vecsei P
• Description:
  An old controversy is resolved as a novel effect: In a rhythmic fashion, aqueous pineal homogenate (APH) enhances, attenuates or leaves unaffected the production of corticosterone by mouse adrenals incubated with pituitary media. All glands stem from the same circadian stage in these (isophasic) studies on 72 female CD2F1 mice, standardized for two weeks in L 0600-1800 and D 0600-1800. Every 4 hours during a 24-hour span, 12 mice were killed. Pineals were removed for the preparation of APH and stored at 4 degrees C. Hypothalami, pituitaries and adrenals were removed, bisected and placed in wells containing 1 ml Krebs-Ringer buffer (K). at 4 degrees C, until incubation. At each circadian stage, bisected adrenals were incubated with 95% O2 and 5% CO2 at 37 +/- 1 degrees C for 5 hours, with K only or with the addition of 0.05 IU ACTH 1-17 or APH or with isophasic pituitary or hypothalamic preincubation media with and without APH or muscle. Media were stored at -20 degrees C until corticosterone RIA. A circadian rhythm (p<0.05) characterized corticosterone production after stimulation by the pituitary alone or with APH. The overall modulatory effect of APH is an increased circadian amplitude of adrenal corticosterone production, in response to the isophasic pituitary. [Description provided by NIOSH]
• Subjects:
  Animals Circadian Rhythm Occupational Health Safety
• Keywords:
  Adrenal Adrenocortical Hormones Animal Studies Author Keywords: Pineal Biological Rhythms Circadian Circadian Rhythms Corticosteroids Corticosterone Heterophasic Sequential Incubation Hormone Activity In Vitro Study Isophasic Sequential Incubation Modulation Mouse Pituitary Pituitary Glands

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• ISSN:
  0361-9230
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; Minnesota ; OSHA Region 5 ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• Issue:
  4
• NIOSHTIC Number:
  nn:20060780
• Citation:
  Brain Res Bull 1983 Apr; 10(4):559-565
• Contact Point Address:
  Dr. Franz Halberg, University of Minnesota, Department of Laboratory Medicine and Pathology, 420 Washington Avenue, S.E., Minneapolis, MN 55455
• Federal Fiscal Year:
  1983
• Performing Organization:
  University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
• Peer Reviewed:
  True
• Start Date:
  19791201
• Source Full Name:
  Brain Research Bulletin
• End Date:
  19880301
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bbd88af9df0d8e5f7230c22f70f9b72bf57e47babdf8a24fb3c32414368fd245578d57eed8a9dc04e88b61127d22a3246baf3e386059ce787dfa7b230189f6f7
• Download URL:
  https://stacks.cdc.gov/view/cdc/224600/cdc_224600_DS1.pdf
• File Type:
  [PDF - 809.35 KB ]