Effects of 4-Tertiary Butyl Catechol on Glutathione-Metabolizing Enzymes In Vivo and In Vitro

Details

• Personal Author:
  Epstein WL ; Fukuyama K ; Gellin GA ; Kawashima T ; Yonemoto K
• Description:
  Intermediates of pheomelanin in tissue cultured B16 melanoma cells were analyzed by high performance liquid chromatography, and reduced glutathione (GSH), L-dopa, 2-[(L)-S-cysteinyl]-L-dopa (2-SCD) and 5-[(L)-S-cysteinyl]-L-dopa (5-SCD) were quantified. The effects of 4-tertiary butylcatechol (TBC), an antioxidant which causes skin depigmentation, on the levels of the intermediate were then examined. A concentration of 10(-4) M TBC increased the intracellular levels of GSH, 2-SCD and 5-SCD, whereas the L-dopa level was unchanged. The time-course of the increased intermediates corresponded to the elevation of glutathione-metabolizing enzyme activities previously reported by Kawashima et al. [J. invest. Derm. 82, 53 (1984)] in the same cell line exposed to 10(-4) M TBC. The findings establish chemical evidence that TBC stimulates pheomelanogenesis in melanocytes. [Description provided by NIOSH]
• Subjects:
  Antioxidants Biochemistry Enzymes Occupational Health Safety Skin
• Keywords:
  Dermatology Enzyme Activity In Vitro Studies In Vivo Studies
• ISSN:
  0022-202X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  53-56
• Volume:
  82
• Issue:
  1
• NIOSHTIC Number:
  nn:20060685
• Citation:
  J Invest Dermatol 1984 Jan; 82(1):53-56
• Federal Fiscal Year:
  1984
• Performing Organization:
  University of California San Francisco, San Francisco, California
• Peer Reviewed:
  True
• Start Date:
  19770701
• Source Full Name:
  Journal of Investigative Dermatology
• End Date:
  19890630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:12a2fa666410095f9ca6a8d8bb92305b3564613dc570d3b378a0c15e40ba907bc1f17e48c5e4ec96fe2d34751f82d7231353908145e5d04ebae666f1acb05256
• Download URL:
  https://stacks.cdc.gov/view/cdc/224524/cdc_224524_DS1.pdf
• File Type:
  [PDF - 1.89 MB ]