Stimulation of Pheomelanogenesis in Cultured B16 Melanoma Cells by 4-Tertiary Butylcatechol

Details

• Personal Author:
  Castagnoli N Jr. ; Epstein WL ; Fukuyama K ; Gellin GA ; Halldin MM ; Kawashima T
• Description:
  Intermediates of pheomelanin in tissue cultured B16 melanoma cells were analyzed by high performance liquid chromatography, and reduced glutathione (GSH), L-dopa, 2-[(L)-S-cysteinyl]-L-dopa (2-SCD) and 5-[(L)-S-cysteinyl]-L-dopa (5-SCD) were quantified. The effects of 4-tertiary butylcatechol (TBC), an antioxidant which causes skin depigmentation, on the levels of the intermediate were then examined. A concentration of 10(-4) M TBC increased the intracellular levels of GSH, 2-SCD and 5-SCD, whereas the L-dopa level was unchanged. The time-course of the increased intermediates corresponded to the elevation of glutathione-metabolizing enzyme activities previously reported by Kawashima et al. [J. invest. Derm. 82, 53 (1984)] in the same cell line exposed to 10(-4) M TBC. The findings establish chemical evidence that TBC stimulates pheomelanogenesis in melanocytes. [Description provided by NIOSH]
• Subjects:
  Antioxidants Biochemistry Enzymes Neoplasms Occupational Health Safety Skin Skin Diseases
• Keywords:
  Enzyme Activity Skin Cancer Skin Lesions Skin Tests
• ISSN:
  0006-2952
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  34
• Issue:
  19
• NIOSHTIC Number:
  nn:20060460
• Citation:
  Biochem Pharmacol 1985 Oct; 34(19):3517-3521
• Contact Point Address:
  Kimie Fukuyama, Department of Dermatology, University of California, San Francisco, San Francisco, CA 94143, U.S.A.
• Federal Fiscal Year:
  1986
• Performing Organization:
  University of California San Francisco, San Francisco, California
• Peer Reviewed:
  True
• Start Date:
  19770701
• Source Full Name:
  Biochemical Pharmacology
• End Date:
  19890630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0529d1d5d537202ea502315897bf93a77c381ca5cee84e6e561c624f52910cdbb20f534618eec03f9f5a08b9f4a5f6661a51a80bac5bd27ba3ba4388b25ec062
• Download URL:
  https://stacks.cdc.gov/view/cdc/224338/cdc_224338_DS1.pdf
• File Type:
  [PDF - 410.97 KB ]