Effects of 4-Tertiary Butyl Catechol on Glutathione-Metabolizing Enzymes In Vivo and In Vitro

Details

• Personal Author:
  Epstein WL ; Fukuyama K ; Gellin GA ; Kawashima T ; Yonemoto K
• Description:
  The effects of noncytotoxic doses of 4-tertiary-butyl-catechol (98293) (TBC) on glutathione-S-transferase (GST) activity in the skin of male, 2 month old Uscd-mice and B16-murine-melanoma cells in culture were investigated. These effects were studied in relation to changes in the activities of glutathione-reductase (GR) and gamma- glutamyl-transpeptidase (GGT) which are involved in pheomelanogenesis. Electron microscopic findings in the in-vivo studies demonstrated an altered form of melanosomes, classified as pheomelanosomes, in the TBC treated animals. Ear tissues demonstrated increased activities for all enzymes tested following TBC application. These increases were 27, 19, and 35 percent for GR, GST, and GGT, respectively. In-vitro studies demonstrated a dose dependent decrease in the eumelanin content. GR activity in TBC treated cells demonstrated a 12 percent elevation already at 12 hours. This continued to be higher than controls throughout the study period. No significant difference in GGT was noted at 12 hours, but it began to elevate at 24 hours, reaching a 26 percent increase after 48 hours. GST also did not increase during the first 12 hours, but showed a 25 percent increase by 48 hours after exposure. The authors suggest that these findings may aid in the construction of an experimental model to investigate the molecular action of other more commonly used chemical depigmenting agents. [Description provided by NIOSH]
• Subjects:
  Antioxidants Enzymes Laboratories Occupational Health Safety
• Keywords:
  Catechols Enzyme-activity In-vitro-studies In-vivo-studies Laboratory-animals NIOSH-Grant Pigmentation-disorders
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-28
• NIOSHTIC Number:
  nn:00181273
• NTIS Accession Number:
  PB88-248349
• Citation:
  Department of Dermatology, University of California, San Francisco, California, 1988 Jan; :1-28
• Contact Point Address:
  Dermatology University of California Department of Dermatology San Francisco, Calif 94143
• CAS Registry Number:
  4-tert-Butylcatechol (CAS RN 98-29-3)
• Federal Fiscal Year:
  1988
• Performing Organization:
  University of California San Francisco, San Francisco, California
• Peer Reviewed:
  False
• Start Date:
  19770701
• Source Full Name:
  Department of Dermatology, University of California, San Francisco, California
• End Date:
  19890630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a71670cb65cdd8c52d998a65cff9e2e1233bb113e4206401961f06598ddd4c4aa2fdcf2e1b9406f74b2fcdf57a51950a0792280e9fb7fcd56a2d05d83e6076dd
• Download URL:
  https://stacks.cdc.gov/view/cdc/217739/cdc_217739_DS1.pdf
• File Type:
  [PDF - 630.55 KB ]