Hypoxia Potentiates Killing of Hepatocyte Monolayers by Leukotrienes, Hydroperoxyeicosatetraenoic Acids, or Calcium Ionophore A23187

Details

• Personal Author:
  Bendix M ; Bosterling B ; Trudell JR
• Description:
  Potentiation of chemical toxicity by hypoxia was studied in confluent hepatocyte monolayers. Addition of either hydroperoxyarachidonic acid (50 ug), leukotriene C4 (10 ug), or calcium ionophore A23187 (1.8 ug) to hepatocyte monolayers followed by incubation in 2% oxygen for 24 h killed 95% of the hypoxic cells, but was without effect on the normoxic cells. The greater than 10-fold increase in toxicity of A23187 suggests that hypoxic cells are less able to regulate intracellular calcium. The increased toxicity of hydroperoxyarachidonic acid and leukotriene C4 may be due to a related reduction in activity of protective enzymes. [Description provided by NIOSH]
• Subjects:
  Biochemistry Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Hypoxia Cell Killing Cellular Effects Hepatocytes Hydroperoxyeicosatetraenoic Acid Hypoxia Ionophore A23187 Leukotriene Oxygen Levels Toxic Effects
• ISSN:
  0006-3002
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  338-341
• Volume:
  803
• Issue:
  4
• NIOSHTIC Number:
  nn:20060641
• Citation:
  Biochim Biophys Acta 1984 Apr; 803(4):338-341
• Contact Point Address:
  James R. Trudell, Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, U.S.A.
• CAS Registry Number:
  Calcium (CAS RN 7440-70-2) ; Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1984
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Biochimica et Biophysica Acta
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4b4ba7cee6a290f8752c0529ec2920bb961da03f6bb5d4a56e9ed313ef3b776fddced9a571b4f370eb96eb714e66d77a1e0c2f2dbba14fd39f0b1ee85ec7d187
• Download URL:
  https://stacks.cdc.gov/view/cdc/224491/cdc_224491_DS1.pdf
• File Type:
  [PDF - 305.50 KB ]