Oxygen Concentration-Dependent Metabolism of Leukotriene B4 by Hepatocyte Monolayers

Details

• Personal Author:
  Costa AK ; Gut J ; Trudell JR
• Description:
  Leukotriene B4 was found to be metabolized by rat hepatocyte monolayers at a rate that was linear with increasing substrate concentration from 74 to 740 nM leukotriene B4. The rates of metabolism were dependent on the O2 concentration and were 315, 213, 80, and 36 pmol leukotriene B4 per min per nmol cytochrome P-450 at 20% (212 micro M), 4% (42.5 micro M), 2% (21.2 micro M), and 1% (10.6 micro M) O2, respectively. The metabolic rate was not linear with respect to O2 concentration; however, half maximal rate occurred at 4% O2, an O2 concentration found in the pericentral region of normally oxygenated liver. These results suggest that in vivo conditions of hypoxia or ischemia that lead to blood O2 concentrations less than 4% may drastically decrease hepatic clearance of leukotriene B4. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal Studies Author Keywords: Leukotriene B4 Cellular Effects Cytochrome P-450 Hepatic Clearance Hepatocytes Hypoxia Oxygen Levels Rat Hepatocyte Toxic Effects
• ISSN:
  0006-3002
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  194-199
• Volume:
  878
• Issue:
  2
• NIOSHTIC Number:
  nn:20060279
• Citation:
  Biochim Biophys Acta 1986 Sep; 878(2):194-199
• Contact Point Address:
  James R. Trudell, Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305
• CAS Registry Number:
  7112-Mercaptoethanol (CAS RN 60-24-2) ; Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1986
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Biochimica et Biophysica Acta
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e6124a4db1750535d5a0551c887bdbd932b49cafbf3bfeb6072162547a43a5b1618384ba2bd9981bb14b17dd5ba9df62f6894c376cd8046e3fc15a9b3be38459
• Download URL:
  https://stacks.cdc.gov/view/cdc/224230/cdc_224230_DS1.pdf
• File Type:
  [PDF - 522.08 KB ]