Interaction of Hypoxia and Carbon Tetrachloride Toxicity in Hepatocyte Monolayers

Details

• Personal Author:
  Costa AK ; Trudell JR
• Description:
  The toxicity of carbon tetrachloride (CCl4) in monolayer cultures of primary hepatocytes was investigated at oxygen concentrations that prevail in the liver under conditions that range from normoxia to hypoxia: 0.5, 1, 2, and 20% O2. CCl4 was administered in the vapor phase at concentrations that produce aqueous concentrations at 37 degrees C of 0.4, 2.0, and 4.0 mM. Damage was assayed by leakage of aspartate transaminase and the inclusion of Trypan Blue immediately after the 2-hr incubation and after an additional 6-hr incubation in 20% O2. Only in the case of 0.5% O2 and 4 mM CCl4 were the monolayers damaged (18%) immediately after the 2-hr exposure; all other exposed cells were undamaged at that time point and the dose response of cell death as a function of CCl4 and oxygen concentration was not evident until the 6-hr time point. The monolayers exposed to 4 mM CCl4 and 1, 2, or 20% O2 exhibited little immediate damage but were all 100% dead 6 hr later. The monolayers exposed to 2 mM CCl4 and 0.5, 1, 2, or 20% O2 were 53, 48, 40, and 22 +/- 2% dead after 6 hr, respectively. These results suggest that effects of CCl4 exposure, for example alterations in the function or synthesis of essential proteins, require several hours to affect cell viability. [Description provided by NIOSH]
• Subjects:
  Biochemistry Lung Lung Diseases Occupational Health Pathology Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Carbon Tetrachloride Cellular Effects Hepatocytes Hypoxia Oxygen Levels Proteins Toxic Effects
• ISSN:
  0014-4800
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  183-192
• Volume:
  50
• Issue:
  2
• NIOSHTIC Number:
  nn:20060047
• Citation:
  Exp Mol Pathol 1989 Apr; 50(2):183-192
• CAS Registry Number:
  Carbon tetrachloride (CAS RN 56-23-5) ; Oxygen (CAS RN 7782-44-7)
• Federal Fiscal Year:
  1989
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Experimental and Molecular Pathology
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7cb1640133c4c954fb1cc832d018084af29716f3a9dd629999a8acab33693710d90041e965c38d1601b7337edfb73f808ab4566d0c986b1b2a3f2dd018fb18a6
• Download URL:
  https://stacks.cdc.gov/view/cdc/224080/cdc_224080_DS1.pdf
• File Type:
  [PDF - 1.98 MB ]