Ultrastructural Studies of Barrier Restoration in Epidermis of Hairless Mice Following Dimethyl Sulfoxide Application

Details

• Personal Author:
  Shackleford JM ; Yielding KL
• Description:
  Sixteen hairless mice were studied by transmission electron microscopy to determine the ultrastructural changes in epidermal burner restoration following topical applications of dimethyl sulfoxide (DMSO) to hack skin. Samples of experimental skin were examined at 30 min, 1 h, 2 h and 3 h after the initial DMSO treatment and compared with that of control animals. At earlier time periods (30 min-1 h) the DMSO treated epidermis showed greatly expanded intercellular spaces, disrupted desmosomal attachments, and desmosomal remnants lying free within the intercellular space. Mitochondria contained droplets of lipoid material which reached maximum size in the spinous and granular layers. Cytoplasmic vacuoles were particularly prominent in the 30-min samples. Large numbers of membrane coaling granules (MCG) emerged in the 1 h specimens. The MCG were laden with numerous electron-dense inner membranes which crisscrossed at various angles. Many of the MCG were observed in the process of fusion with plasma membranes of granular cells adjacent to the stratum corneum. At 2 h a process of recovery from the effects of DMSO was evident and at 3 h there was little or no difference between experimental and control epidermis. [Description provided by NIOSH]
• Subjects:
  Animals Occupational Health Safety Skin Skin Absorption
• Keywords:
  Animal Studies Cellular Effects Dermal Exposures Electron Microscopy Skin Absorption Skin Exposure Sulfoxides
• ISSN:
  0303-6987
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Alabama ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  259-268
• Volume:
  11
• Issue:
  4
• NIOSHTIC Number:
  nn:20060610
• Citation:
  J Cutan Pathol 1984 Aug; 11(4):259-268
• Contact Point Address:
  John M. Shackleford, Ph.D., Department of Anatomy, College ot Medicine, Mobile, Alabama 36688, USA
• CAS Registry Number:
  Dimethyl sulfoxide (CAS RN 67-68-5)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Cutaneous Pathology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ea760e76465e84f5c30be6fdb5c952a8d916a228042c32d1d7191cabbc87caa9b2bcb439915f5047a3d1be1ecb3b228bbb6a45578700a00004fc5ac027610543
• Download URL:
  https://stacks.cdc.gov/view/cdc/224467/cdc_224467_DS1.pdf
• File Type:
  [PDF - 6.39 MB ]