Ethyl Acetate Extraction of Spin-Trapped Free Radicals: A Reevaluation

Details

• Personal Author:
  Trudell JR
• Description:
  We have suggested the use of ethyl acetate for extraction of hydroxyl or superoxide radical adducts of the spin trap phenyl N-tert-butyl nitrone (PBM). The technique produced EPR spectra with narrow line widths, the radical adducts were more stable, and there were sufficiently large differences between the isotropic nitrogen hyperfine coupling constant (aN) and the beta hydrogen coupling constant (aHbeta) for both the hydroxyl and superoxide radical adducts to allow their simultaneous quantitation in mixtures. However, Kalyanaraman, Mottley, and Mason have suggested that our assignments of aN and aHbeta were incorrect and that extraction of spin-trapped adducts into ethyl acetate is not as useful as we had proposed. This paper demonstrates that their objections are unfounded and are based on a computational error that they made when they attempted to calculate the hyperfine splittings in their spectra. [Description provided by NIOSH]
• Subjects:
  Free Radicals Occupational Health Safety
• Keywords:
  5,5-Dimethyl-I-pyrroline-N-oxide Analytical Methods Author Keywords: EPR DMPO ESR Ethyl Acetate Free Radicals Hydroxyl Groups Hydroxyl Radical PBN Phenyl N-tert-butyl Nitrone Spin Trapping Superoxide

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• ISSN:
  0891-5849
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  133-136
• Volume:
  3
• Issue:
  2
• NIOSHTIC Number:
  nn:20060259
• Citation:
  Free Radic Biol Med 1987 Jan; 3(2):133-136
• CAS Registry Number:
  5,5-Dimethyl-1-pyrroline N-oxide (CAS RN 3317-61-1) ; Ethyl acetate (CAS RN 141-78-6)
• Federal Fiscal Year:
  1987
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Free Radical Biology and Medicine
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c66990612da7870eebc6b09f0af6f97defcbcabed9e0fbe40517107580c23f4e6822d4bfcd758c2d060d084ef497e24429d59028dde4a890b7ea7b04e7aceda1
• Download URL:
  https://stacks.cdc.gov/view/cdc/224216/cdc_224216_DS1.pdf
• File Type:
  [PDF - 315.69 KB ]