Antioxidant Properties of Pyrrolidine Dithiocarbamate and Its Protection Against Cr(VI)-Induced DNA Strand Breakage

Details

• Personal Author:
  Ding M ; Leonard, Stephen S. ; Shi X ; Wang S
• Description:
  Pyrrolidine dithiocarbamate (PDTC) is considered an antioxidant and is frequently used to study the role of free radical reacions in various biological processes and against free radical-induced cellular injuries. However, its antioxidant properties are not characterized. In this study, electron spin resonance (ESR) was used to investigate the antioxidant potential of PDTC with hydroxyl radical (OH) and superoxide anion radicals (O2-). The Fenton reaction [Fe(II) + H2O2 --> Fe(II) + OH + OH-)] and xanthine and xanthine oxidase were used as sources of OH and O2- radicals, respectively. The results show that PDTC effectively scavenges OH radicals with a reaction rate constant of approximately 2.73 x 10(10) M(-1) s(-1), which is comparable to other efficient OH radical scavengers, such as ascorbate and glutathione. PDTC is also able to scavenge O2- radicals. Through its antioxidant properties, PDTC protects against Cr(VI)-induced DNA strand breakage. [Description provided by NIOSH]
• Subjects:
  Antioxidants Free Radicals Occupational Health Safety
• Keywords:
  Antioxidant Antioxidation Author Keywords: Pyrrolidine Dithiocarbamate Cellular Reactions Cr(VI) DNA Damage Free Radical Generation Free Radicals Hydroxyl Groups Hydroxyl Radical Superoxide Radical

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• ISSN:
  0091-7370
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  209-216
• Volume:
  30
• Issue:
  2
• NIOSHTIC Number:
  nn:20020807
• Citation:
  Ann Clin Lab Sci 2000 Apr; 30(2):209-216
• Contact Point Address:
  Xianglin Shi, Ph.D., Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Mail Stop B167, Morgantown, WV 26505, USA
• Email:
  xas0@cdc.gov
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Clinical and Laboratory Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:42303537e36c5a512cd0398c13ec135c0875ce29f9d995531d3ddcdd1e1bebfa937994ecfc781c3b9f5e6996130a40a3ccfb995618bcb1042d89bc4f7f69ff80
• Download URL:
  https://stacks.cdc.gov/view/cdc/198472/cdc_198472_DS1.pdf
• File Type:
  [PDF - 2.07 MB ]