Effect of Chlorogenic Acid on Hydroxyl Radical

Details

• Personal Author:
  Castranova, Vincent ; Cosma G ; Gardner H ; Vallyathan V ; Zang LY
• Description:
  Chlorogenic acid (CGA) is considered to act as an antioxidant. However, the inhibitory effects of CGA on specific radical species are not well understood. Electron spin resonance (ESR) in combination with spin trapping techniques was utilized to detect free radicals. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was used as a spin trapping reagent while the Fenton reaction was used as a source of hydroxyl radical (*OH). We found that CGA scavenges *OH in a dose-dependent manner. The kinetic parameters, IC50 and Vmax, for CGA scavenging of *OH were 110 and 1.27 microM/sec, respectively. The rate constant for the scavenging of *OH by CGA was 7.73 x 10(9) M(-1) sec(-1). Our studies suggest that the antioxidant properties of CGA may involve a direct scavenging effect of CGA on *OH. [Description provided by NIOSH]
• Subjects:
  Antioxidants Free Radicals Occupational Health Safety
• Keywords:
  Author Keywords: Chlorogenic Acid ESR Free-radicals Hydroxyl-groups Hydroxyl Radical In-vitro-studies In-vivo-studies Spin Trapping
• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  205-210
• Volume:
  247
• Issue:
  1
• NIOSHTIC Number:
  nn:20023076
• Citation:
  Mol Cell Biochem 2003 May; 247(1-2):205-210
• Contact Point Address:
  Exposure Assessment Branch and Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA
• Email:
  laz7@cdc.gov
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:41273af53f195bf544eca5f26231d4c5c048e418171ed35b2262f80fe6753e2e824cb5138c2f85c662a8968bf61e99cdf4d85d9aea4fc199f9a45f8c700e545c
• Download URL:
  https://stacks.cdc.gov/view/cdc/199659/cdc_199659_DS1.pdf
• File Type:
  [PDF - 80.22 KB ]