Suppression of Bone Marrow Stromal Cell Function by Benzene and Hydroquinone Is Ameliorated by Indomethacin

Details

• Personal Author:
  Gaido KW ; Wierda D
• Description:
  Administration of benzene to mice will inhibit bone marrow stromal cell-supported hemopoiesis in culture. Hydroquinone, a major metabolite of benzene, will cause a similar inhibition of stromal cell function in vitro. Stromal cells produce both an inducer (colony-stimulating factor) and an inhibitor (prostaglandin E2; PGE2) of hemopoiesis. This research was conducted to determine if prostaglandin synthesis is involved in the suppression of stromal cell function by benzene and hydroquinone. Male B6C3F1 mice were administered benzene (100 mg/kg), indomethacin (1 mg/kg), or benzene plus indomethacin twice a day for 4 consecutive days. On Day 5 bone marrow cells were removed to determine the effect of treatment. In a second series of experiments mouse bone marrow stromal cells in culture were treated with hydroquinone (10-7 to 10-4 m), indomethacin (10-6 m), or a combination of hydroquinone plus indomethacin. Stromal cell function was based on the ability of the treated stromal cells to support granulocyte/monocyte colony development in coculture. The results demonstrated that preadministration of indomethacin in vivo ameliorated benzene-induced inhibition of bone marrow stromal cell function. In vitro, indomethacin ameliorated hydroquinone toxicity to stromal cell function. Benzene administration in vivo induced elevated PGE2 in bone marrow samples which were prevented by preadministration of indomethacin. However, hydroquinone in vitro did not induce a consistent increase in PGE2 levels. These results suggested that toxicity to stromal cells was not due solely to increased prostaglandin synthetase activity. [Description provided by NIOSH]
• Subjects:
  Animals Benzene Hydroquinones Occupational Health Safety Toxicology
• Keywords:
  Animal Studies Bone Marrow Cellular Effects Cellular Function Hydroquinone Pharmacology Toxic Effects
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  378-390
• Volume:
  89
• Issue:
  3
• NIOSHTIC Number:
  nn:20060224
• Citation:
  Toxicol Appl Pharmacol 1987 Jul; 89(3):378-390
• CAS Registry Number:
  Benzene (CAS RN 71-43-2) ; Hydroquinone (CAS RN 123-31-9)
• Federal Fiscal Year:
  1987
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19830401
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19900831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:254f2abbd766a881ae6491bc1c62062110e9533b71b47d93dfef9e0746b196466748fb046aabc1b1f18297463d562045510a200e2afa18ab890e6363745f842e
• Download URL:
  https://stacks.cdc.gov/view/cdc/224193/cdc_224193_DS1.pdf
• File Type:
  [PDF - 762.47 KB ]