Lead Treatment of Myelin Basic Protein (MBP) Enhances the Antigenicity of MBP in CBA/J Mice

Details

• Personal Author:
  El-Fawal HAN ; Snyder CA ; Waterman S
• Description:
  Environmental pollutants have been suspected of playing a role in the pathogenesis of nervous system autoimmune disease, such as multiple sclerosis (MS). Lead (Pb) is known to impact on both the immune and nervous systems. In the latter, Pb exposure has been shown to result in autoantibody titers to nervous system proteins, including MBP. We hypothesize that Pb aggravates neural autoimmune disease by enhancing the antigenicity of neurotypic proteins, such as MBP. To test this hypothesis, MBP was incubated for 24h with Pb acetate and then passed through a Sephadex column to remove unbound Pb. Mice received 3 inoculations on days 0, 14 and 28 with Saline + Incomplete Freunds Adjuvant OFA), MBP + IFA, or Pb altered MBP + IFA. Anti-MBP isotypes lgM and lgG were measured in sera, by ELISA, on day 42. Saline + IFA controls, unlike earlier studies in rats, had background titers of anti-MBP (100%). MBP + IFA did not produce a significant increase in anti-MBP titers above background (104%). However Pb altered MBP + IFA resulted in a significant increase in both lgM (126%) and lgG (440%). This exceeded the lgM and lgG titers (105 and 163%, respectively) produced by a 6wk p.o. exposure to 450 ppm Pb. This latter study indicates that Pb enhances the production of anti-MBP isotypes. The present study shows that Pb enhances the antigenicity of MBP. Other immunological mechanisms, e.g. Pb induction of T cell help in precipitating neural autoimmune disease, are under investigation. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Exposure Environmental Pollution Immune System Lead Nervous System Nervous System Diseases Occupational Health Safety Toxicology
• Keywords:
  Animal Studies Autoimmune Diseases Environmental Pollution Immune System Nervous System Disorders Pathogenesis
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  1
• NIOSHTIC Number:
  nn:20060142
• Citation:
  Toxicologist 1994 Mar; 14(1):291
• CAS Registry Number:
  Lead (CAS RN 7439-92-1)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Mount Sinai School of Medicine, New York
• Peer Reviewed:
  False
• Start Date:
  19920701
• Source Full Name:
  The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
• End Date:
  19970630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:91035dc77779af263a3acc9189c0d3774e86562cfc36dbd4e7f804846754bc9184a02cec1b68e15c30904e1298852c28c2cf773036f1b582ebaa8e3442e66616
• Download URL:
  https://stacks.cdc.gov/view/cdc/224145/cdc_224145_DS1.pdf
• File Type:
  [PDF - 219.91 KB ]