Inhibition of Protein Kinase C Phosphorylation by Mono- and Divalent Cations

Details

• Personal Author:
  Costa AK ; Csernansky CA ; Trudell JR
• Description:
  The effect of a matrix of concentrations of Ca2+ (0.01, 0.1, 0.5, 5 mM), Mg2+ (0.2, 0.5, 1, 2, 5, 10 mM), and Na+ (50, 100, 150 mM) on the phosphorylation of histone H-1 by protein kinase C was measured in the presence of 5 mol % diacylglycerol and Mg-ATP in both phosphatidylserine micelles and liposomes formed from a 1:4 mixture of phosphatidylserine and phosphatidylcholine. Monovalent cations (150 mM) reduced activity by 60 and 84% in the micelle and liposome assay systems, respectively. Inhibition was also observed with 5 mM Ca2+ and 10 mM Mg2+. The phosphorylating activity was compared with computer calculations of the negative electrostatic potentials (Psi o) of the phospholipid membranes in the presence of the cations. [Description provided by NIOSH]
• Subjects:
  Biochemistry Lipids Occupational Health Safety
• Keywords:
  Biochemical Analysis Biochemical Effects Phospholipids Protein Biochemistry Proteins
• ISSN:
  0006-291X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  45-50
• Volume:
  162
• Issue:
  1
• NIOSHTIC Number:
  nn:20060022
• Citation:
  Biochem Biophys Res Commun 1989 Jul; 162(1):45-50
• Federal Fiscal Year:
  1989
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Biochemical and Biophysical Research Communications
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eddb95be6c9a66867e51e0436e5fc0c6c28d9b6667e333acbdca918b51829a457751f4c06e36bcc5d3e0ba51c240c8101c49b58b31095e03b0c3eee27bb49d80
• Download URL:
  https://stacks.cdc.gov/view/cdc/224059/cdc_224059_DS1.pdf
• File Type:
  [PDF - 435.53 KB ]