Elucidating Regulatory Processes of Intense Physical Activity by Multi-Omics Analysis

Details

• Personal Author:
  Allen KA ; Bloodsworth KJ ; Bramer LM ; Burnum-Johnson KE ; Claborne D ; Clark KA ; Gao Y ; Gritsenko MA ; Kim Y-M ; Kyle JE ; Montes F ; Munoz N ; Nakayasu ES ; Navarro KM ; Nicora CD ; Paurus VL ; Stratton KG ; Teeguarden J ; Tietje G ; Weitz KK
• Description:
  Background: Physiological and biochemical processes across tissues of the body are regulated in response to the high demands of intense physical activity in several occupations, such as firefighting, law enforcement, military, and sports. A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work environment. Methods: To study regulatory processes in intense physical activity simulating real-life conditions, we performed a multi-omics analysis of three biofluids (blood plasma, urine, and saliva) collected from 11 wildland firefighters before and after a 45 min, intense exercise regimen. Omics profiles post- versus pre-exercise were compared by Student's t-test followed by pathway analysis and comparison between the different omics modalities. Results: Our multi-omics analysis identified and quantified 3835 proteins, 730 lipids and 182 metabolites combining the 3 different types of samples. The blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy demands. The urine analysis showed a strong, concomitant regulation of 6 out of 8 identified proteins from the renin-angiotensin system supporting increased excretion of catabolites, reabsorption of nutrients and maintenance of fluid balance. In saliva, we observed a decrease in 3 pro-inflammatory cytokines and an increase in 8 antimicrobial peptides. A systematic literature review identified 6 papers that support an altered susceptibility to respiratory infection. Conclusion: This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility, suggesting that caution against respiratory diseases could benefit workers on highly physical demanding jobs. [Description provided by NIOSH]
• Subjects:
  Biochemistry Energy Metabolism Immune System Lipids Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Biofluids Biochemical Effects Human Performance Immune Reaction Immunity Intense Exercise Metabolism Metabolites Multi-omics Analysis Physical Exercise Physiological Response Protein Biochemistry Proteins Respiratory Diseases

  More +
• ISSN:
  2054-9369
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; Oregon ; OSHA Region 10 ; OSHA Region 3 ; OSHA Region 8 ; OSHA Region 9 ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  WSD - Western States Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  48
• Volume:
  10
• NIOSHTIC Number:
  nn:20068638
• Citation:
  Mil Med Res 2023 Oct; 10:48
• Contact Point Address:
  Ernesto S. Nakayasu, Biological Sciences Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA, 99352, USA
• Email:
  Ernesto.Nakayasu@pnnl.gov
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Military Medical Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8e78142413efa079b42b72a4d083bd43b3920b4fed0dd15d0636f47d60a7258b911b3c29c307cf27027b085634136096f1e01ed9dfbedced0eadc4a3317cf457
• Download URL:
  https://stacks.cdc.gov/view/cdc/207239/cdc_207239_DS1.pdf
• File Type:
  [PDF - 4.45 MB ]