In Vitro Cytotoxicity and Potential Carcinogenesis of Copper Oxide Nanoparticles

Details

• Personal Author:
  Aldinger, Jeffrey A. ; Arnold, James E. ; BarberT ; Ding M
• Description:
  Occupational exposures to copper nanoparticles and fine particles have been reported to be harmful to human health. There is a possible risk of cancer among industrial workers. Copper (II) oxide (CuO) nanoparticles have not, to date, been extensively examined for potential carcinogenic effects. Mechanisms of CuO-induced pathogenesis, the effect of CuO nanoparticles on cyto-toxicity and carcinogenic effects were investigated. Using the 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide cellular dehydrogenase activity (MTT Assay) assay, CuO nanoparticles showed a dose-dependent reduction of the dehydrogenase activity (DHA) in the JB6 cell culture medium indicating the cytotoxicity of CuO. The potential carcinogenesis effects of CuO were also examined. The data indicates that chronically exposed human lung epithelial JB6 cells to low-dose CuO resulted in neoplastic transformation using the soft agar assay. In vitro genotoxicity test, single cell gel electrophoresis assay (SCGE; also called the comet assay), was used for the detection of the mutagenic potential of nano- and fine-sized CuO. The results suggested that both nano- and fine-sizes CuO induce DNA damage. CuO nanoparticles exhibit greater genotoxicity than fine particles. Understanding complex mechanisms associated with these events may provide insights into the initiation and progression of CuO-induced carcinogenesis. [Description provided by NIOSH]
• Subjects:
  Biological Assay Carcinogens Cells, Cultured Cytotoxins Lung Mutagens Occupational Exposure Occupational Health Respiratory System Safety Toxicology

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• Keywords:
  Bioassays Carcinogenesis Carcinogenicity Cell Cultures Cell Culture Techniques Cellular Effects Chronic Exposure Copper Dust Copper Oxide Cytotoxic Effects Cytotoxicity DNA Damage Dose Response Genotoxic Effects Genotoxicity Humans In Vitro Study Lung Cells Mutagenicity Nanoparticles Nanotechnology Nanotoxicology Neoplastic Transformation Oxides Pathogenesis Toxic Effects Ultrafine Particles

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  17
• Volume:
  174
• Issue:
  1
• NIOSHTIC Number:
  nn:20058845
• Citation:
  Toxicologist 2020 Mar; 174(1):17
• CAS Registry Number:
  Copper oxide (CuO) (CAS RN 1317-38-0)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 59th Annual Meeting and ToxExpo, March 15-19, 2020, Anaheim, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1567b450f19aa511b7ad38717e99fd0e6729407ace73e1df08f56c1e05f8933dc8735f13fdd6f7e92ba8b89c4efc48594824de8363b7360351c7443bf6158bba
• Download URL:
  https://stacks.cdc.gov/view/cdc/223230/cdc_223230_DS1.pdf
• File Type:
  [PDF - 524.11 KB ]