Perfluorocarbon Nanoemulsions Enhance Therapeutic siRNA Delivery in the Treatment of Pulmonary Fibrosis

Details

• Personal Author:
  Bailey KL ; Ding L ; Knoell DL ; Mosley DD ; Oupický D ; Romanova S ; Sil D ; Tang S ; Tang W ; Wyatt TA ; Yu A
• Description:
  Local pulmonary administration of therapeutic siRNA represents a promising approach to the treatment of lung fibrosis, which is currently hampered by inefficient delivery. Development of perfluorooctylbromide (PFOB) nanoemulsions as a way of improving the efficiency of pulmonary polycation-based delivery of siRNA is reported. The results show that the polycation/siRNA/PFOB nanoemulsions are capable of efficiently silencing the expression of STAT3 and inhibiting chemokine receptor CXCR4 - two validated targets in pulmonary fibrosis. Both in vitro and in vivo results demonstrate that the nanoemulsions improve mucus penetration and facilitate effective cellular delivery of siRNA. Pulmonary treatment of mice with bleomycin-induced pulmonary fibrosis shows strong inhibition of the progression of the disease and significant prolongation of animal survival. Overall, the study points to a promising local treatment strategy of pulmonary fibrosis. [Description provided by NIOSH]
• Subjects:
  Fluorocarbons Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Laboratory Animals Lung Fibrosis Nanomaterials Nanostructures Pulmonary System Therapeutic Agents
• ISSN:
  2198-3844
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  8
• NIOSHTIC Number:
  nn:20064302
• Citation:
  Adv Sci 2022 Mar; 9(8):2103676
• Contact Point Address:
  David Oupický, Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA
• Email:
  david.oupicky@unmc.edu
• CAS Registry Number:
  Perfluorooctyl bromide (CAS RN 423-55-2)
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Advanced Science
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:869f64422fb05db42f965139c8448769bc61aca0bf2b95246a51e7a699175ecd2482ceb0f854a94f04913b5d3a158cc382693249cbb01624ae1cf45b75be1407
• Download URL:
  https://stacks.cdc.gov/view/cdc/222793/cdc_222793_DS1.pdf
• File Type:
  [PDF - 7.65 MB ]