Effect of Nanoparticle Surface Coating on Cell Toxicity and Mitochondria Uptake

Details

• Personal Author:
  Bentley K ; DeLouise LA ; Mortensen LJ ; Ravichandran S ; Zheng H
• Description:
  We report on the effect of surface charge and the ligand coating composition of CdSe/ZnS core/shell quantum dot (QD) nanoparticles on human keratinocyte toxicity using fluorescent microscopy, flow cytometry, transmission electron microscopy. Two commonly reported positive charged (cysteamine, polyethylenimine) and two negative charged (glutathione, dihydrolipoic acid) ligands were studied. The QDs were fully characterized by UV-vis absorption spectroscopy, fluorescence emission spectroscopy, dynamic light scattering and zeta potential. Differences in surface coatings and charges were evaluated against cellular uptake, ROS generation, cytotoxicity, and mitochondrial targeting. Results show that the negative charged QDs coated with GSH exhibit excellent water solubility, high quantum yield and low cytotoxicity. Ligand composition is more important in ROS generation than surface charge whereas surface charge is an important driver of cytotoxicity. Most importantly we observe the selective accumulation of glutathione coated QDs in vesicles in the mitochondria matrix. This observation suggests a new strategy for developing mitochondria-targeted nanomaterials for drug/gene delivery. [Description provided by NIOSH]
• Subjects:
  Occupational Health Safety Skin Toxicology
• Keywords:
  Author Keywords: Quantum Dots Cellular Effects Dermal Absorption Endocytosis Humans Intracellular Localization Keratinocytes Mitochondria Nanoparticles Nanotoxicology Quantum Dots Skin Tests

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• ISSN:
  1550-7033
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  155-166
• Volume:
  13
• Issue:
  2
• NIOSHTIC Number:
  nn:20058511
• Citation:
  J Biomed Nanotechnol 2017 Feb; 13(2):155-166
• Contact Point Address:
  Lisa A. DeLouise, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York 14642, USA
• Email:
  Lisa_DeLouise@urmc.rochester.edu
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of Rochester, New York
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Journal of Biomedical Nanotechnology
• End Date:
  20140831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a42c647d73403e5932b43f86a0f4f82953802f3767ba5881dafded284cda198a9c53218811085c9df26ccc3077300613c24c9f37af96596b5b0e1ce8c1c55899
• Download URL:
  https://stacks.cdc.gov/view/cdc/221588/cdc_221588_DS1.pdf
• File Type:
  [PDF - 1.46 MB ]