Quantification of Human Skin Barrier Function and Susceptibility to Quantum Dot Skin Penetration

Details

• Personal Author:
  DeLouise LA ; Mortensen LJ ; Ravichandran S
• Description:
  In this study, we utilize in vivo human skin and a viable ex-vivo human skin model to investigate the effect of a commercial depilatory agent on barrier function. Tape stripping was used as a positive control. The magnitude of skin barrier was quantified by measuring transepidermal water loss values on in vivo human skin and transepithelial electrical resistance measurements and tissue histology on ex vivo skin. The susceptibility to carboxylated quantum dot penetration through ex vivo skin was investigated using fluorescent microcopy analysis of microtomed skin sections and flow cytometry to quantify quantum dot association with live epidermal cells. Results show that depilatory treatment modifies the outside-in barrier sufficiently to allow quantum dots to penetrate the stratum corneum but to a lesser extent than tape stripping. The implications of these finding are discussed. [Description provided by NIOSH]
• Subjects:
  Occupational Health Safety Skin Toxicology
• Keywords:
  Author Keywords: Quantum Dots Depilatory Agent Dermal Absorption Nanoparticles Nanotoxicology Skin Exposure Skin Penetration Skin Tests Tape Stripping
• ISSN:
  1743-5390
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  5
• Issue:
  4
• NIOSHTIC Number:
  nn:20057825
• Citation:
  Nanotoxicology 2011 Dec; 5(4):675-686
• Contact Point Address:
  Dr Lisa A. DeLouise, Department of Dermatology, University of Rochester, Box 697, 601 Elmwood Ave, Lab 6-6823, Rochester, NY 14642, USA
• Email:
  lisa_delouise@urmc.rochester.edu
• Federal Fiscal Year:
  2012
• Performing Organization:
  University of Rochester, New York
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Nanotoxicology
• End Date:
  20140831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d17f70371c85b557792c3b4369c7cbc481cf09afdd8750692f3c9dff5f7c74b8ae1ce85254209a2bb1e1b0096c9ba4b69d35433fae98c81bdcd96ae7914b8a20
• Download URL:
  https://stacks.cdc.gov/view/cdc/215042/cdc_215042_DS1.pdf
• File Type:
  [PDF - 801.81 KB ]