Sample Preparation Method for Visualization of Nanoparticulate Captured on Mixed Cellulose Ester Filter Media by Enhanced Darkfield Microscopy and Hyperspectral Imaging

Details

• Personal Author:
  Brenner SA ; Eastlake AC ; Neu-Baker NM
• Description:
  A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential worker exposure. Current best-known methods include transmission electron microscopy (TEM) coupled with energy dispersive x-ray spectroscopy (EDS) for elemental identification. TEM-EDS is considerably time-, cost-, and resource-intensive, which may prevent timely health and safety recommendations and corrective actions. A rapid screening method is currently being explored using enhanced darkfield microscopy with hyperspectral imaging (EDFM-HSI). For this approach to be effective, rapid, and easy, sample preparation that is amenable to the analytical technique is needed. Here, we compare the sample preparation steps for mixed cellulose ester (MCE) filter media specified in NIOSH Method 7400-Asbestos and Other Fibers by Phase Contrast Microscopy (PCM)-against a new method, which involves saturation of the filter media with acetone. NIOSH Method 7400 was chosen as a starting point since it is an established technique for preparing transparent MCE filters for optical microscopy. Limitations in this method led to the development and comparison of a new method. The new method was faster, easier, and rendered filters more transparent, resulting in improved visualization and analysis of nanomaterials via EDFM-HSI. This new method is suitable for a rapid screening protocol due to its speed, ease of use, and the improvement in image acquisition and analysis. [Description provided by NIOSH]
• Subjects:
  Acetone Cellulose Occupational Health Particulate Matter Safety
• Keywords:
  Author Keywords: Acetone Vaporization Engineered Nanomaterial Exposure Assessment Fibers Filters Imaging Techniques Microscopy Nanomaterials Nanoparticles Nanotechnology Phase Contrast Microscopy Risk Assessment

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• ISSN:
  1059-910X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; Ohio ; OSHA Region 2 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  EID - Education and Information Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  82
• Issue:
  6
• NIOSHTIC Number:
  nn:20054925
• Citation:
  Microsc Res Tech 2019 Jun; 82(6):878-883
• Contact Point Address:
  N. M. Neu-Baker, College of Nanoscale Science, Nanobioscience Constellation, State University of New York (SUNY) Polytechnic Institute, College of Nanoscale Science, New York
• Email:
  nneu@sunypoly.edu
• CAS Registry Number:
  Acetone (CAS RN 67-64-1)
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Microscopy Research and Technique
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2bcb3972da238744a3d2486d84758321b990976d85fb81f5d3e31210ad19513757103a3aa4df95e728956448e78cbbe086e311e8883dde0b5fbbd493248447f2
• Download URL:
  https://stacks.cdc.gov/view/cdc/217335/cdc_217335_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]