Organic Barn Dust Inhibits Surfactant Protein D Production Through Protein Kinase-C Alpha Dependent Increase of GPR116

Details

• Personal Author:
  DeVasure JM ; Kirychuk SA ; Schneberger D ; Wyatt TA
• Description:
  Prolonged exposure to organic barn dusts can lead to chronic inflammation and a broad range of lung problems over time, mediated by innate immune mechanisms. The immune surfactant or collectin surfactant protein D (SP-D) is a crucial multifunctional innate immune receptor. Little work to date has examined the effect of such collectins in response to organic dusts. We provide evidence here that agricultural organic dusts can inhibit mRNA and protein expression of SP-D in a human alveolar epithelial cell line, and an in vivo mouse model. This inhibition was not a result of lipopolysaccharide (LPS) or peptidoglycans, the two most commonly cited immune active components of these dusts. We further show that inhibition of the signaling molecule protein kinase C alpha (PKCa) can reverse this inhibition implicating it as a mechanism of SP-D inhibition. Examination of the SP-D regulatory receptor GPR116 showed that its mRNA expression was increased in response to dust and inhibited by blocking PKCa, implicating it as a means of inhibiting SP-D in the lungs in response to organic dusts. This reduction shows that organic barn dust can reduce lung SP-D, thus leaving workers potentially at risk for a host of pathogens. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Enzymes Immune System Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar Cells Chronic Inflammation Dust Exposure Enzyme Activity Immune Reaction Immunology Lung Disease Organic Dusts Proteins
• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  12
• NIOSHTIC Number:
  nn:20054853
• Citation:
  PLoS One 2018 Dec; 13(12):e0208597
• Contact Point Address:
  Todd A. Wyatt, Department of Internal Medicine, Pulmonary, Critical Care, Sleep & Allergy Division, University of Nebraska Medical Center, Omaha, Nebraska, United States of America
• Email:
  twyatt@unmc.edu
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  PLoS One
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8feb9871822e9f1a5899ccae7a97e2e84a997642fb953a69e0d2855996ac647d8a27f862c414f1c9954fe41dd8178a798266aee0ea63b8afc79f8b89dacf1138
• Download URL:
  https://stacks.cdc.gov/view/cdc/217271/cdc_217271_DS1.pdf
• File Type:
  [PDF - 796.80 KB ]