Post-Injury and Resolution Response to Repetitive Inhalation Exposure to Agricultural Organic Dust in Mice

Details

• Personal Author:
  Ailts I ; Heires AJ ; Nelson AJ ; Nordgren TM ; Poole JA ; Romberger DJ ; Staab E ; Warren KJ ; West WW ; Wyatt TA
• Description:
  Inhalation of organic dusts in agricultural environments causes airway inflammatory diseases. Despite advances in understanding the airway response to dust-induced inflammation, less is known about the transition from lung injury to repair and recovery. The objective of this study was to define the post-inflammation homeostasis events following organic dust-induced lung injury. Using an established protocol, mice were intranasally treated with swine confinement facility organic dust extract (ODE) daily for 3 weeks (repetitive exposure) or treated daily with ODE for 3 weeks followed by no treatment for 1-4 weeks (recovery period) whereupon lavage fluid, lung tissue, and sera were processed. During recovery period, a significant decrease was observed in ODE-induced neutrophil levels after 1 week, lymphocytes at 2 weeks, and macrophages at 4 weeks in the lavage fluid. ODE-induced lung cellular aggregates and bronchiolar compartment inflammation were diminished, but persisted for 4 weeks post-injury. Alveolar inflammation resolved at 3 weeks. ODE-induced lung neutrophils were cleared by 3 weeks, B-cells by 2 weeks, and CD3+CD4+ and CD3+CD8+ T cells by 4 week recovery period. Collectively, these results identify important processes during recovery period following agricultural dust-induced inflammation, and present possible strategies for improving lung repair and resolution. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Immune System Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar Cells Amphiregulin Author Keywords: Lung Dust Exposure Dust Inhalation Exposure Assessment Farm ILC2 Immune Reaction Immunity Inflammation Inhalation Studies Innate Lymphoid Cells Laboratory Animals Lung Disease Lymphocyte Neutrophil Neutrophils Organic Dusts Repair Resolution Swine Barn

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• ISSN:
  2313-576X
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  10
• Volume:
  3
• Issue:
  1
• NIOSHTIC Number:
  nn:20051086
• Citation:
  Safety 2017 Mar; 3(1):10
• Contact Point Address:
  Jill A. Poole, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center (UNMC), 985990 Nebraska Medical Center, Omaha, NE 68198-5990, USA
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Safety
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7f77cd5e4d9df671292662e1a383105d2f4cdb0e7df2ff4136bf9599381ba7463afbe6907e2221a17164c8576b3c1ba5d8932140f17fff1d46dd254feaf72b2e
• Download URL:
  https://stacks.cdc.gov/view/cdc/211212/cdc_211212_DS1.pdf
• File Type:
  [PDF - 3.15 MB ]