Effect of Age, High-Fat Diet, and Rat Strain on Serum Biomarkers and Telomere Length and Global DNA Methylation in Peripheral Blood Mononuclear Cells

Details

• Personal Author:
  Antonini JM ; Boyce GR ; Erdely A ; Kashon M ; Kodali V ; Meighan TG ; Roach KA ; Roberts, Jennifer R. ; Salmen R ; Shoeb M ; Zeidler-Erdely PC
• Description:
  The objective of the current study was to determine if age, diet, and genetic disposition (animal strain) in an animal model had early effects on specific molecular markers in circulating peripheral blood mononuclear cells (PBMCs). Three strains [Sprague-Dawley (SD), Fischer 344 (F344), and Brown-Norway (BN)] of male rats were maintained on a high-fat (HF) or regular diet. Blood was collected at 4, 12, and 24 wk to assess chemistry and to recover PBMCs. Triglycerides and body weight gain increased at all time points in the HF diet group for each strain. Telomere length in PBMCs decreased in the HF diet group compared to the regular diet group up to 24 wk in all strains. Telomere length decreased in PBMCs at 24 wk compared to baseline in all strains, indicating an age-related effect. These findings highlight that diet and age cause changes in PBMCs recovered from different strains of rats. The next tier of studies will examine the contribution of an occupational exposure (e.g., welding fume inhalation) in combination with diet, age, and strain, to assess changes in the molecular responses of isolated PBMCs. In addition, studies involving lifestyle exposure (e.g., tobacco smoke) are in the planning stages and will assess the long-term effects of exposure in our animal model. [Description provided by NIOSH]
• Subjects:
  Age Factors Biomarkers Blood Diet Environmental Exposure Genetics Laboratories Occupational Health Safety
• Keywords:
  Age Factors Biological Effects Blood Cells DNA Damage Environmental Exposure Laboratory Animals
• ISSN:
  2045-2322
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• NIOSHTIC Number:
  nn:20054851
• Citation:
  Sci Rep 2019 Feb; 9:1996
• Contact Point Address:
  James M. Antonini, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, 26505
• Email:
  jag6@cdc.gov
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Scientific Reports
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9bca80df880535a96d888817ff5eef85720e8a8cd51037311acf37ee7b516ffc1fcc1dd89a5a7a7f277f8fbfb0e00d9b758d07eb40e9f93fe7c88d41e5c2f158
• Download URL:
  https://stacks.cdc.gov/view/cdc/217269/cdc_217269_DS1.pdf
• File Type:
  [PDF - 2.14 MB ]