Effect of Age, Diet Restriction and Aminoguanidine on Pentosidine in the Skin of Broiler Breeder Hens

Details

• Personal Author:
  Al-humadi NH ; Igbal M ; Klandorf H ; Probert LL ; Van Dyke K
• Description:
  Non-enigmatic glycosylation contributes to the formation of cross-links which leads to the deterioration in the structure and function of tissue protein. Whether the elevated concentrations of plasma glucose in chickens accelerates the accumulation of cross-links has not been determined. The objectives of this study were to determine the effect of diet restriction (DR) and the cross-linking inhibitor aminoguandine (AG) on the rate of accumulation of the glycoxidation product, pentosidine, in the skin of naturally hyperglycemuc broiler breeder (BB) hens. BB female chicks (ne=450) were randomly assigned to four groups from 8 to 125 wks after hatch. ad libitum (AL), diet restricted (DR); ad libilum and diet restricted groups supplemented with 400 ppm AG each (AL + AG and DR + AG respectively). Skin pentosidine was isolated by HPLC and oxidative stress was measured by luminometery. Results showed that the accumulation of pentosidine increased linearly (P<0.001) with age. DR significantly (P<0.001) retarded the rate of accumulation of pentosidine. The concentration of pentosidine in AL + AG group was comparable with that of DR group and skin pentosidine was lowest (P<0.001) in both DR and DR + AG groups as compared to AL and AL + AG groups. It was concluded that the rate of accumulation of pentosidine can be retarded by DR and AG. In addition, the data supports the view that pentosidine can be used as a biomarker for the study of aging in chickens. [Description provided by NIOSH]
• Subjects:
  Age Factors Animals Biomarkers Diet Laboratories Occupational Health Safety Skin
• Keywords:
  Age-factors Animal-studies Dietary-effects Genotoxic-effects Laboratory-animals Skin-exposure Tissue-disorders
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  12
• Issue:
  5
• NIOSHTIC Number:
  nn:20026393
• Citation:
  FASEB J 1998 Mar; 12(5)(Pt 2)(Suppl):A879
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The FASEB Journal
• Supplement:
  S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9819cd1f28e4f6a715a8daa1161612785146bbf3e80bc2e3b22fc822e6f274eabe19bef86a368b58f436febba52ac648a445c53f2f63e06b47dd4ae33e1edd8d
• Download URL:
  https://stacks.cdc.gov/view/cdc/197266/cdc_197266_DS1.pdf
• File Type:
  [PDF - 332.30 KB ]