Evaluation of Matrix Effects in Quantifying Microbial Secondary Metabolites in Indoor Dust Using Ultraperformance Liquid Chromatograph-Tandem Mass Spectrometer

Details

• Personal Author:
  Jaderson M ; Park, Ju-Hyeong
• Description:
  Background: Liquid chromatography-tandem mass spectrometry (LC-MSMS) for simultaneous analysis of multiple microbial secondary metabolites (MSMs) is potentially subject to interference by matrix components. Methods: We examined potential matrix effects (MEs) in analyses of 31 MSMs using ultraperformance LC-MSMS. Twenty-one dust aliquots from three buildings (seven aliquots/building) were spiked with seven concentrations of each of the MSMs (6.2 pg/mle900 pg/ml) and then extracted. Another set of 21 aliquots were first extracted and then, the extract was spiked with the same concentrations. We added deepoxy-deoxynivalenol (DOM) to all aliquots as a universal internal standard. Ten microliters of the extract was injected into the ultraperformance LC-MSMS. ME was calculated by subtracting the percentage of the response of analyte in spiked extract to that in neat standard from 100. Spiked extract results were used to create a matrix-matched calibration (MMC) curve for estimating MSM concentration in dust spiked before extraction. Results: Analysis of variance was used to examine effects of compound (MSM), building and concentration on response. MEs (range: 63.4%-99.97%) significantly differed by MSM (p < 0.01) and building (p < 0.05). Mean percent recoveries adjusted with DOM and the MMC method were 246.3% (SD 226.0) and 86.3% (SD 70.7), respectively. Conclusion: We found that dust MEs resulted in substantial underestimation in quantifying MSMs and that DOM was not an optimal universal internal standard for the adjustment but that the MMC method resulted in more accurate and precise recovery compared with DOM. More research on adjustment methods for dust MEs in the simultaneous analyses of multiple MSMs using LC-MSMS is warranted. [Description provided by NIOSH]
• Subjects:
  Construction Industry Construction Materials Dust Energy Metabolism Fungi Humidity Hydrocarbons Occupational Health Safety
• Keywords:
  Author Keywords: Dust Sample Construction Materials Dusts Indoor Environments Mass Spectrometry Matrix Effect Metabolites Microbial Molds Secondary Metabolite
• ISSN:
  2093-7911
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  196-204
• Volume:
  10
• Issue:
  2
• NIOSHTIC Number:
  nn:20054313
• Citation:
  Saf Health Work 2019 Jun; 10(2):196-204
• Contact Point Address:
  Ju-Hyeong Park, Respiratory Health Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Safety and Health at Work
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c66ec5a62e9c9a3d1160370ead26876062f06217ed75e40a978f5ff61981097da9f7c0ea0103fbdc33c868b6213a683b41c179b7ee57194159eb6187f48223ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/216904/cdc_216904_DS1.pdf
• File Type:
  [PDF - 834.68 KB ]