U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Adjustment of Matrix Effects in Analysis of 36 Secondary Metabolites of Microbial and Plant Origin in Indoor Floor Dust Using Liquid Chromatography-Tandem Mass Spectrometry

Public Domain


Details

  • Personal Author:
  • Description:
    Exposure to microbial agents in water-damaged buildings is a major public health concern. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become a primary tool for testing environmental samples for microbial secondary metabolites (SMs); however, matrix effects can lead to inaccurate results in exposure assessment. Applying a universal internal standard (ISTD) and a matrix-matched calibration can adjust for matrix effects, as shown by our previous study. However, there are only few isotope-labeled internal standards for SMs available on the market. In this study, we determined the best-performing ISTDs among ten candidates (nine 13C-labeled isotopes and one unlabeled analogue) for each of 36 SMs. We analyzed school floor dust spiked with the 36 SMs to identify the best-performing ISTDs (initial experiment) and examined reproducibility with the selected ISTDs and the same spiked dust (validation 1). We also tested applicability for the selected ISTDs using spiked dust collected from different schools (validation 2). The three experiments showed that 26, 17, and 19 SMs had recoveries within the range 100 +/- 40%. 13C-ochratoxin A and 13C-citrinin were most frequently selected as the best ISTDs for the 36 SMs, followed by deepoxy-deoxynivalenol, 13C-sterigmatocystin, and 13C-deoxynivalenol. Our study shows that using the identified, best-performing analogous ISTDs for those metabolites may improve testing accuracy for indoor dust and help better estimate exposure effects on potential health. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    2075-5309
  • Document Type:
    Text
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Division:
    RHD - Respiratory Health Division
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    13
  • Issue:
    5
  • NIOSHTIC Number:
    nn:20067749
  • Citation:
    Buildings 2023 May; 13(5):1112
  • Contact Point Address:
    Ju-Hyeong Park, Respiratory Health Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA
  • Email:
    gzp8@cdc.gov
  • Federal Fiscal Year:
    2023
  • NORA Priority Area:
    Services
  • Peer Reviewed:
    True
  • Source Full Name:
    Buildings
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:0d2c6276758702b82a6389ca2be9ae1a99ee4cb7d2e573f5b24083597433fb368c47e3acbf7c532d9e16fb9582818483f3fd3d37f554f21a6acd6f192a68a3cd
  • Download URL:
  • File Type:
    Filetype[PDF - 476.82 KB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP