Ambient Particulate Matter Enhances the Pulmonary Allergic Immune Response to House Dust Mite in a BALB/C Mouse Model by Augmenting Th2- and Th17-Immune Responses

Details

• Personal Author:
  Bein KJ ; Castañeda AR ; Hughes HK ; Pinkerton KE ; Smiley-Jewell S ; Vogel CFA
• Description:
  Ambient particulate matter (PM) exacerbates airway inflammation and hyper-reactivity in asthmatic patients. Studies show that PM has adjuvant-like properties that enhance the allergic inflammatory response; however, the mechanisms through which PM enhances these processes remain elusive. The objective of the study was to examine how ambient PM enhances the allergic immune response. Eight-week-old BALB/c mice were sensitized with house dust mite (HDM) or HDM and ambient particulate matter (PM, 2.5 µm; Sacramento, California) to assess how PM modulates the development of adaptive immune responses against allergens. Both groups were challenged with HDM only. Bronchoalveolar lavage (BAL) was analyzed for extent of airway inflammation. Lung tissue was used for histological analysis, mucosubstance quantification, and heme oxygenase-1 (HO-1) localization/quantification. Gene expression was analyzed in whole lung to characterize immune markers of inflammation: cytokines, chemokines, antioxidant enzymes, and transcription factors. Cytokine and chemokine protein levels were quantified in whole lung to confirm gene expression patterns. Compared to HDM-only sensitization, exposure to PM during HDM sensitization led to significant immune cell recruitment into the airway subepithelium, IgE gene expression, mucosubstance production, and Th2-associated cytokine expression. HO-1 levels were not significantly different between the treatment groups. Gene expression profiles suggest that polycyclic aromatic hydrocarbon (PAH) content in PM activated the aryl hydrocarbon receptor (AhR) and enhanced Th17-responses in the mice that received HDM and PM compared to mice that received HDM-only. The findings suggest that PM enhances allergic sensitization via enhancement of Th2-mediated inflammation and that AhR activation by PAHs in PM promotes Th17-immune responses. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Antioxidants Biomarkers Dust Histology Hydrocarbons Hypersensitivity Immune System Laboratories Lung Occupational Health Particulate Matter Polycyclic Aromatic Hydrocarbons Respiratory System Safety

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• Keywords:
  Airborne Particulates Allergens Allergic Reactions Allergy Author Keywords: Allergic Airway Inflammation Cytokines Dusts Gene Expression House Dust Mite Allergen Immune Reaction Laboratory Animals Lung Tissue Particulate Matter Polycyclic Aromatic Hydrocarbons Sensitization Th2 Immunity Th17 Immunity

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• ISSN:
  2051-817X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  6
• Issue:
  18
• NIOSHTIC Number:
  nn:20052917
• Citation:
  Physiol Rep 2018 Sep; 6(18):e13827
• Contact Point Address:
  Kent E. Pinkerton, Department of Pediatrics, Center for Health and the Environment, University of California, Davis, CA 95616
• Email:
  kepinkerton@ucdavis.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Physiological Reports
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b930d97064ae0e05f74bd1ebe3db1996996f692795a6a2580fe1a2af2731de71dfe7cf974b06e6e21543dd2e6a047fcf81006f3befbe698f506d76cdb71a022a
• Download URL:
  https://stacks.cdc.gov/view/cdc/215822/cdc_215822_DS1.pdf
• File Type:
  [PDF - 2.33 MB ]