Immune Response Signatures of Benzo(a)pyrene Exposure in Normal Human Mammary Epithelial Cells in the Absence or Presence of Chlorophyllin

Details

• Personal Author:
  John K ; Keshava C ; Nath J ; Richardson DL ; Weston A
• Description:
  Carcinogenic polycylic aromatic hydrocarbons can alter immune responses. Changes in immune response gene expression profiles in multiple human mammary cell strains exposed to benzo(a)pyrene (BP) (4 µM) in vitro, in the presence or absence of chlorophyllin (5 µM), were observed using Affymetrix gene arrays. Expressions of five immune response genes were altered approximately 3.0-fold by BP exposure and 24 genes by BP in the presence chlorophyllin. In silico pathway analysis revealed altered immune response genes form interactive gene networks with many cellular processes, suggesting their role in a complex multigenic response to toxins. Additionally, it was suggestive of the possible immunomodulatory potential of chlorophyllin apart from various other well-documented mechanisms of action. Gene expression matrices revealed consistent alteration patterns involving IL1B, SECTM1 and CXCL14 on exposure to BP, and IL1RN, CD86, IF144 and GIP2 in the presence of chlorophyllin and BP, suggesting some of these genes might constitute putative immune response biomarkers of PAH exposure. This study has therefore identified a battery of potential immune response biomarkers of PAH exposure, amidst several genes, for future validation studies. [Description provided by NIOSH]
• Subjects:
  Biomarkers Carcinogens Genetics Hazardous Substances Hydrocarbons Immune System Occupational Health Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  Author Keywords: Carcinogens Biological-effects Chemoprevention Chlorophyllin Gene Expression Genetic-factors Immune-reaction Polycyclic-aromatic-hydrocarbons Polycyclic-hydrocarbons Polycyclic Aromatic Hydrocarbons Toxins

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• ISSN:
  1109-6935
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-11
• Volume:
  6
• Issue:
  1
• NIOSHTIC Number:
  nn:20036895
• Citation:
  Cancer Genomics Proteomics 2009 Jan-Feb; 6(1):1-11
• Contact Point Address:
  Joginder Nath, Genetics and Developmental Biology Program, 1120 Agricultural Sciences Building, West Virginia University, Morgantown, WV 26506-6108
• Email:
  jnath@wvu.edu
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  2009
• Peer Reviewed:
  True
• Source Full Name:
  Cancer Genomics & Proteomics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:98a526031e3faed1bf06c592c4ddfdc9fb2096889a8a7c0de9d51503da47dc88dd15496e5aba7e279cfdb550a99d3cc5a1cbbee5e2e16fba01fc77b06f9170af
• Download URL:
  https://stacks.cdc.gov/view/cdc/188125/cdc_188125_DS1.pdf
• File Type:
  [PDF - 424.41 KB ]