Ambient Particulate Matter Activates the Aryl Hydrocarbon Receptor in Dendritic Cells and Enhances Th17 Polarization

Details

• Personal Author:
  Ashwood P ; Bein KJ ; Castañeda AR ; Magaña-Méndez A ; Pinkerton KE ; Vogel CFA ; Yang HT
• Description:
  The objective of this study was to explore the role of the aryl hydrocarbon receptor (AhR) in ambient particulate matter (PM)-mediated activation of dendritic cells (DCs) and Th17-immune responses in vitro. To assess the potential role of the AhR in PM-mediated activation of DCs, co-stimulation, and cytokine expression, bone marrow (BM)-derived macrophages and DCs from C57BL/6 wildtype or AhR knockout (AhR-/-) mice were treated with PM. Th17 differentiation was assessed via co-cultures of wildtype or AhR-/- BMDCs with autologous naive T cells. PM2.5 significantly induced AhR DNA binding activity to dioxin responsive elements (DRE) and expression of the AhR repressor (AhRR), cytochrome P450 (CYP) 1A1, and CYP1B1, indicating activation of the AhR. In activated (OVA sensitized) BMDCs, PM2.5 induced interleukin (IL)-1β, CD80, CD86, and MHC class II, suggesting enhanced DC activation, co-stimulation, and antigen presentation; responses that were abolished in AhR deficient DCs. DC-T cell co-cultures treated with PM and lipopolysaccharide (LPS) led to elevated IL-17A and IL-22 expression at the mRNA level, which is mediated by the AhR. PM-treated DCs were essential in endowing T cells with a Th17-phenotype, which was associated with enhanced expression of MHC class II and cyclooxygenase (COX)-2. In conclusion, PM enhances DC activation that primes naive T cell differentiation towards a Th17-like phenotype in an AhR-dependent manner. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Antigens Blood Enzymes Hydrocarbons Immune System Macrophages Occupational Health Particulate Matter Polycyclic Aromatic Hydrocarbons Safety

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• Keywords:
  AhR Airborne Particulates Aryls Author Keywords: Aryl Hydrocarbon Receptor Cytokines DCs Dendritic Cells Enzyme Activity Gene Regulation Immune Reaction In Vitro Study PAHs Particulate Matter PM Polycyclic Aromatic Hydrocarbons Th17 Cells

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  85-96
• Volume:
  292
• NIOSHTIC Number:
  nn:20051743
• Citation:
  Toxicol Lett 2018 Aug; 292:85-96
• Contact Point Address:
  Christoph F. A. Vogel, Center for Health and the Environment, University of California, Davis, Bldg 3792, Rm 129, Old Davis Road, Davis, California 95616, USA
• Email:
  cfvogel@ucdavis.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Toxicology Letters
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c734f06d8a8ff3e7dea74c6ad181d250882afdaa400d128fb573ce097b3d0a3547a88d111eccf3bcf8f420291b8151687de2af626d26cc811ceedba2182bcbf0
• Download URL:
  https://stacks.cdc.gov/view/cdc/211533/cdc_211533_DS1.pdf
• File Type:
  [PDF - 1.67 MB ]