Molecular Barriers to Zoonotic Transmission of Prions

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Barria, Marcelo A. ; Balachandran, Aru ; Morita, Masanori ; Kitamoto, Tetsuyuki ; Barron, Rona ; Manson, Jean ; Knight, Richard ; Ironside, James W. ; Head, Mark W.
• Description:
  The risks posed to human health by individual animal prion diseases cannot be determined a priori and are difficult to address empirically. The fundamental event in prion disease pathogenesis is thought to be the seeded conversion of normal prion protein to its pathologic isoform. We used a rapid molecular conversion assay (protein misfolding cyclic amplification) to test whether brain homogenates from specimens of classical bovine spongiform encephalopathy (BSE), atypical BSE (H-type BSE and L-type BSE), classical scrapie, atypical scrapie, and chronic wasting disease can convert normal human prion protein to the abnormal disease-associated form. None of the tested prion isolates from diseased animals were as efficient as classical BSE in converting human prion protein. However, in the case of chronic wasting disease, there was no absolute barrier to conversion of the human prion protein.
• Subjects:
  Bovine Spongiform Encephalopathy BSE Cell-free System Chronic Wasting Disease CJD Creutzfeldt-Jakob Disease In Vitro Assay Prion Disease Prions Protein Misfolding Diseases Research Scrapie Zoonoses

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• Source:
  Emerg Infect Dis. 20(1):88-97.
• Document Type:
  Journal Article
• Volume:
  20
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:92a8f6e933c5df1481931c32ab6b91c405995a21a837b38474506a87589b9405
• Download URL:
  https://stacks.cdc.gov/view/cdc/21524/cdc_21524_DS1.pdf
• File Type:
  [PDF - 1.07 MB ]