Evaluation of Aneuploidy and DNA Damage in Human Spermatozoa: Applications in Field Studies

Details

• Personal Author:
  Evenson DP ; Perreault SD ; Robbins WA ; Rubes J ; Selevan SG
• Description:
  With the goal of incorporating measures of sperm nuclear integrity in an epidemiology study, semen samples from young Czech men were analysed for sperm aneuploidy and sperm chromatin structure in addition to routine measures of sperm production and quality. The exposure in question was to high seasonal air pollution containing reactive polyaromatic hydrocarbons potentially capable of affecting spermatogenesis and damaging sperm DNA. The sperm aneuploidy assay uses fluorescence in situ hybridization to label selected sperm chromosomes; as applied in this study, the sex chromosomes (X,Y) and chromosome 8 were targeted. The sperm chromatin structure assay detects sperm nuclei with increased susceptibility to denaturation, a feature that is associated with DNA damage. Logistically, these assays were relatively easy to incorporate into the study design. The aneuploidy assay provided information suggesting that exposure to high levels of air pollution may increase the risk of sperm aneuploidy and that it is important to control for exposure to cigarette smoke and/or alcohol in such studies. The sperm chromatin structure assay provided valuable baseline information about Czech semen donors and data suggestive of an adverse effect of smoking and air pollution on spermatozoa that merits further investigation. [Description provided by NIOSH]
• Subjects:
  Air Pollution Chromosomes Occupational Health Safety Smokers
• Keywords:
  Air Pollution Aneuploidy Author Keywords: Air Pollution Chromatin Chromosome Damage DNA Damage Human Humans PAH Polynuclear Aromatic Hydrocarbons Smoking Spermatozoa

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• ISSN:
  0303-4569
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; District of Columbia ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 8 ; OSHA Region 9 ; South Dakota
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  247-254
• Volume:
  32
• Issue:
  4
• NIOSHTIC Number:
  nn:20058001
• Citation:
  Andrologia 2000 Sep; 32(4-5):247-254
• Contact Point Address:
  Dr S. D. Perreault, 2525 NC Highway 54, Durham, NC 27713, USA
• Email:
  darney.sally@epa.gov
• Federal Fiscal Year:
  2000
• Performing Organization:
  University of California, School of Public Health, Los Angeles, CA
• Peer Reviewed:
  True
• Start Date:
  19990701
• Source Full Name:
  Andrologia
• End Date:
  20040630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6ff5c56a4b669e55f50ce3188316c56cff52a626fc003f7e95ead3a1a42b78055632ae0cfba9e371bcd2f01ad78517f121b3f9a164035b48e08444243f1d4576
• Download URL:
  https://stacks.cdc.gov/view/cdc/215194/cdc_215194_DS1.pdf
• File Type:
  [PDF - 104.32 KB ]