Urinary Levels of Insecticide Metabolites and DNA Damage in Human Sperm

Details

• Personal Author:
  Barr DB ; Bennett DH ; Duty SM ; Hauser R ; Herrick, Robert F. ; Meeker JD ; Ryan L ; Singh NP
• Description:
  BACKGROUND: Members of the general population are exposed to non-persistent insecticides at low levels. The present study explored whether environmental exposures to carbaryl and chlorpyrifos are associated with DNA damage in human sperm. METHODS: Subjects (n=260) were recruited through a Massachusetts infertility clinic. Individual exposures were measured as spot urinary metabolite concentrations of chlorpyrifos [3,5,6-trichloro-2-pyridinol (TCPY)] and carbaryl [1-naphthol (1N)], adjusted using specific gravity. Sperm DNA integrity was assessed by neutral comet assay and reported as comet extent, percentage DNA in comet tail (Tail%) and tail distributed moment (TDM). RESULTS: A statistically significant increase in Tail% was found for an interquartile range (IQR) increase in both 1N [coefficient=4.1; 95% confidence interval (CI) 1.9-6.3] and TCPY (2.8; 0.9-4.6), while a decrease in TDM was associated with IQR changes in 1N (-2.2; -4.9 to 0.5) and TCPY (-2.5; -4.7 to -0.2). A negative correlation between Tail% and TDM was present only when stratified by comet extent, suggesting that Tail% and TDM may measure different types of DNA damage within comet extent strata. CONCLUSIONS: Environmental exposure to carbaryl and chlorpyrifos may be associated with increased DNA damage in human sperm, as indicated by a change in comet assay parameters. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Environmental Exposure Environmental Pollution Hazardous Substances Insecticides Men Occupational Health Safety Smokers Urine
• Keywords:
  Author Keywords: Comet Assay Carbaryl Chlorpyrifos DNA Damage Drugs Environmental Exposure Environmental Pollution Exposure Humans Metabolism Metabolites Pesticides Physiology Regression Analysis Smoking Spermatozoa Toxins Urine Chemistry

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• ISSN:
  0268-1161
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Massachusetts ; OSHA Region 1 ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  19
• Issue:
  11
• NIOSHTIC Number:
  nn:20057810
• Citation:
  Hum Reprod 2004 Nov; 19(11):2573-2580
• Contact Point Address:
  Russ Hauser, Department of Environmental Health, Occupational Health Program, Harvard School of Public Health, Building 1 Room 1405, 665 Huntington Avenue, Boston, MA 02115
• Email:
  rhauser@hohp.harvard.edu
• CAS Registry Number:
  3,5,6-Trichloro-2-pyridinol (CAS RN 6515-38-4) ; Carbaryl (CAS RN 63-25-2) ; Chlorpyrifos (CAS RN 2921-88-2)
• Federal Fiscal Year:
  2005
• Performing Organization:
  Harvard School of Public Health
• Peer Reviewed:
  True
• Start Date:
  20030701
• Source Full Name:
  Human Reproduction
• End Date:
  20050630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:97f510f295d5cacee8171c6e1f03503627f8f2cae5534b79b625549f775f47233ca02d447a405ccf5d86874bbff6a05bcfafbec0da8809d422dbfb89351a67d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/215035/cdc_215035_DS1.pdf
• File Type:
  [PDF - 197.02 KB ]