A Urinary Metabolite of Phenanthrene as a Biomarker of Polycyclic Aromatic Hydrocarbon Metabolic Activation in Workers Exposed to Residual Oil Fly Ash

Details

• Personal Author:
  Carmella SG ; Christiani, David C. ; Hecht SS ; Kim JY ; Mukherjee S ; Rodrigues EG
• Description:
  Residual oil fly ash is a chemically complex combustion product containing a significant component of potentially carcinogenic transition metals and polycyclic aromatic hydrocarbons (PAH). Various biomarkers of PAH exposure have been investigated previously, most notably 1-hydroxypyrene (1-OHP), in urine. In this study, we assessed the utility of r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetrahydrophenanthrene (trans, anti-PheT), a metabolite of phenanthrene, to detect occupational PAH exposure. Urine samples collected across the workweek were analyzed for 1-OHP and trans, anti-PheT in boilermakers (n = 20) exposed to residual oil fly ash. Median baseline urinary trans, anti-PheT concentrations were 0.50 microg/g creatinine in current tobacco smokers and 0.39 microg/g creatinine in nonsmokers. Median baseline urinary 1-OHP concentrations in smokers and nonsmokers were 0.31 and 0.13 microg/g creatinine, respectively. To study further the effect of smoking exposure on the urinary PAH markers, urinary cotinine was used. Although urinary trans, anti-PheT and 1-OHP concentrations were correlated (Spearman r = 0.63; P < 0.001) for all subjects, the regression coefficient between log-transformed trans, anti-PheT and log 1-OHP was statistically significant only for subjects with low levels of urinary cotinine or for nonsmokers. Each 1-unit increase in log 1-OHP was associated with a 0.77-unit increase (95% confidence interval, 0.45-1.09) in log trans, anti-PheT in subjects with low levels of urinary cotinine (P < 0.001). In these subjects, dichotomized occupational exposure status was a significant predictor of log trans, anti-PheT (P = 0.02) but not of log 1-OHP (P = 0.2). In conclusion, we found that urinary trans, anti-PheT was detected in levels comparable with 1-OHP in occupationally exposed workers, particularly nonsmokers. This study shows that urinary trans, anti-PheT may be an effective biomarker of uptake and metabolic activation of PAHs. [Description provided by NIOSH]
• Subjects:
  Air Pollution Aluminum Silicates Biomarkers Energy Metabolism Ferrous Compounds Metals Occupational Health Safety Smokers Tobacco Products Urine Workplace

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• Keywords:
  Air Pollution Carbon Iron Metabolites Nickel Compounds Nitrogen Compounds Phenanthrenes Polychlorinated Aromatic Hydrocarbons Silicates Smoking Sulfates Tobacco Urine Chemistry Vanadium Compounds Work Environment Workers

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• ISSN:
  1055-9965
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  3
• NIOSHTIC Number:
  nn:20057332
• Citation:
  Cancer Epidemiol Biomark Prev 2005 Mar; 14(3):687-692
• Contact Point Address:
  David C. Christiani, Occupational Health Program, Department of Environmental Health, Harvard School of Public Health, Building I, Room 1402, 665 Huntington Avenue, Boston, MA 02115
• Email:
  dchristi@hsph.harvard.edu
• CAS Registry Number:
  1-Hydroxypyrene (CAS RN 5315-79-7) ; Carbon (CAS RN 7440-44-0) ; Iron (CAS RN 7439-89-6) ; Nickel (CAS RN 7440-02-0) ; Phenanthrene (CAS RN 85-01-8) ; Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  2005
• Performing Organization:
  Harvard School of Public Health, Boston, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  19980701
• Source Full Name:
  Cancer Epidemiology, Biomarkers & Prevention
• End Date:
  20030630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4c3f51f2431652114ca2d5d9eb2f0c9046e730bc0ff2293a16c99ca420099a2950b4a0d7829c5e446d06f6dc3c3a04ae465da2efb12336c746346fe445000e3b
• Download URL:
  https://stacks.cdc.gov/view/cdc/214720/cdc_214720_DS1.pdf
• File Type:
  [PDF - 205.98 KB ]