In Utero Exposure to Benzo [A] Pyrene Results in Ovarian Follicular Depletion in F1 MutaTM Mouse

Details

• Personal Author:
  Meharda S
• Description:
  In contrast to gametogenesis in males, oocytes in females are not continually replenished during reproductive life. Exposure to ovotoxicants during or after embryogenesis can lead to irreversible ovarian dysgenesis or premature ovarian failure (POF). Benzo [a] pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) and a component of air pollution. PAHs are formed as a result of incomplete combustion of organic materials and are ubiquitous environmental contaminants. Found in cigarette smoke, polycyclic aromatic hydrocarbons are "toxic to and destructive to oocytes in several animal test systems." Men and women who smoke have decreased fertility. Our study for the first time uses the lacZ transgenic MutaTMMouse, mouse model to study female reproductive consequences of prenatal exposure to BaP Pregnant transgenic MutaMouse were treated with 0, 10, 20 or 40 mg/kg/d BaP orally from gestational day 7 through 16, which represents the major period of organogenesis. The F1 female offspring were euthanized at 10 wk. of age and ovaries were processed for differential ovarian follicle counting. Transplacental BaP exposure caused very significant dose-related decreases in primordial, primary and secondary follicles in the ovaries of F1 MutaMice. There was prominent ovarian tissue destruction at the higher doses of BaP exposure. Our results show that maternal exposure to BaP during gestation is highly damaging to the developing ovaries of the F1 female offspring in Muta mice as it is in other mouse strains that have been studied. Therefore, this model is an excellent model not only for observing the mutation effects but also for showing the reproductive effects. Our findings in the mouse are relevant to humans, as ovarian development in humans and mice is very similar. Increasing evidence regarding PAH exposure and its harmful effects leads us to believe that additional regulations to reduce PAH exposure are needed at the government level. In addition, campaigns to increase awareness among the general population about PAHs are needed, as "Prevention is the key". [Description provided by NIOSH]
• Subjects:
  Animals Occupational Health Pregnancy Safety Urogenital System Women
• Keywords:
  Animal Studies B(a)P Benzo(a)pyrene Fertility Polychlorinated Aromatic Hydrocarbons Prenatal Exposure Reproductive Effects
• Publisher:
  University of California Irvine
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Thesis
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-45
• NIOSHTIC Number:
  nn:20055913
• Citation:
  Irvine, CA: University of California Irvine, 2016 Mar; :1-45
• Federal Fiscal Year:
  2016
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  In utero exposure to benzo [a] pyrene results in ovarian follicular depletion in F1 MutaTM mouse
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6ec96024ff7f4b970f710b0eb6564a09eac4489db44a53c557518399838bc466e72c189add9d9fe88e9c59e21c049fc164bc4d93f590b6a4b713ab133065f935
• Download URL:
  https://stacks.cdc.gov/view/cdc/213667/cdc_213667_DS1.pdf
• File Type:
  [PDF - 903.74 KB ]