Ovarian Effects of Prenatal Exposure to Benzo[a]pyrene: Roles of Embryonic and Maternal Glutathione Status

Details

• Personal Author:
  Banda M ; Luderer U ; McKim KL ; Myers MB ; Ortiz L ; Parsons BL
• Description:
  Females deficient in the glutamate cysteine ligase modifier subunit (Gclm) of the rate-limiting enzyme in glutathione synthesis are more sensitive to ovarian follicle depletion and tumorigenesisby prenatal benzo[a]pyrene (BaP) exposure than Gclm+/+ mice. We investigated effects of prenatal exposure to BaP on reproductive development and ovarian mutations in Kras, a commonly mutated gene in epithelial ovarian tumors. Pregnantmice were dosed from gestational day 6.5 through 15.5 with 2 mg/kg/day BaP or vehicle. Puberty onset occurred 5 days earlier in F1 daughters of all Gclm genotypes exposed to BaP compared to controls. Gclm+/- F1 daughters of Gclm+/- mothers and wildtype F1 daughters of wildtype mothers had similar depletion of ovarian follicles following prenatal exposure to BaP, suggesting that maternal Gclm genotype does not modify ovarian effects of prenatal BaP. We observed no BaP treatment or Gclm genotype related differences in ovarian Kras codon 12 mutations in F1 offspring. [Description provided by NIOSH]
• Subjects:
  Occupational Health Pregnancy Safety Urogenital System Women
• Keywords:
  Author Keywords: Polycyclic Aromatic Hydrocarbon B(a)P Benzo(a)pyrene Glutathione Kras Mutation Ovarian Follicles Prenatal Exposure Puberty Reproductive Effects
• ISSN:
  0890-6238
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; California ; OSHA Region 6 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  187-195
• Volume:
  69
• NIOSHTIC Number:
  nn:20055905
• Citation:
  Reprod Toxicol 2017 Apr; 69:187-195
• Contact Point Address:
  Ulrike Luderer, Center for Occupational and Environmental Health, University of California Irvine, 100 Theory Drive, Suite 100, Irvine, CA 92617-1830, United States
• Email:
  uluderer@uci.edu
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8) ; Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Reproductive Toxicology
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4663eef0f9376f25be659fe5383f471b33310a5df6ca852022bbba96e74ca2cdcd101c8b7de8d5565cc596b5af05d84512731c697f5e461d30a592b63d1c112c
• Download URL:
  https://stacks.cdc.gov/view/cdc/213659/cdc_213659_DS1.pdf
• File Type:
  [PDF - 1.85 MB ]