Colorimetric and Electrochemical Bacteria Detection Using Printed Paper- and Transparency-Based Analytic Devices

Details

• Personal Author:
  Adkins JA ; Bisha B ; Boehle K ; Chamberlain B ; Friend C ; Henry CS
• Description:
  The development of transparency-based electrochemical and paper-based colorimetric analytic detection platforms is presented as complementary methods for food and waterborne bacteria detection from a single assay. Escherichia coli and Enterococcus species, both indicators of fecal contamination, were detected using substrates specific to enzymes produced by each species. beta-galactosidase (beta-gal) and beta-glucuronidase (beta-glucur) are both produced by E. coli, while beta-glucosidase (beta-gluco) is produced by Enterococcus spp. Substrates used produced either p-nitrophenol (PNP), o-nitrophenol (ONP), or p-aminophenol (PAP) as products. Electrochemical detection using stencil-printed carbon electrodes (SPCEs) was found to provide optimal performance on inexpensive and disposable transparency film platforms. Using SPCEs, detection limits for electrochemically active substrates, PNP, ONP, and PAP were determined to be 1.1, 2.8, and 0.5 µM, respectively. A colorimetric paper-based well plate system was developed from a simple cardboard box and smart phone for the detection of PNP and ONP. Colorimetric detection limits were determined to be 81 µM and 119 µM for ONP and PNP respectively. While colorimetric detection methods gave higher detection limits than electrochemical detection, both methods provided similar times to positive bacteria detection. Low concentrations (101 CFU/mL) of pathogenic and nonpathogenic E. coli isolates and (100 CFU/mL) E. faecalis and E. faecium strains were detected within 4 and 8 h of pre-enrichment. Alfalfa sprout and lagoon water samples served as model food and water samples, and while water samples did not test positive, sprout samples did test positive within 4 h of pre-enrichment. Positive detection of inoculated (2.3 × 102 and 3.1 × 101 CFU/mL or g of E. coli and E. faecium, respectively) sprout and water samples tested positive within 4 and 12 h of pre-enrichment, respectively. [Description provided by NIOSH]
• Subjects:
  Chemistry, Analytic Colorimetry Environmental Pollution Enzymes Hazardous Substances Hazardous Waste Microbiology Occupational Health Phenol Safety
• Keywords:
  Amino Compounds Analytical Chemistry Analytical Instruments Analytical Methods Animal Waste Bacteria Detectors Electrochemical Properties Electrochemical Reactions Environmental Contamination Filters Food Contaminants Hazardous Waste Humans Materials Testing Microorganisms Nitrophenols Pathogens Performance Tests Phenols Sampling Sampling Methods Sensors Water Sampling

  More +
• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8 ; Wyoming
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  89
• Issue:
  6
• NIOSHTIC Number:
  nn:20052786
• Citation:
  Anal Chem 2017 Mar; 89(6):3613-3621
• Contact Point Address:
  Charles S. Henry, Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, United States
• Email:
  Chuck.Henry@colostate.edu
• CAS Registry Number:
  2-Nitrophenol (CAS RN 88-75-5) ; 4-Aminophenol (CAS RN 123-30-8) ; 4-Nitrophenol (CAS RN 100-02-7)
• Federal Fiscal Year:
  2017
• Performing Organization:
  Colorado State University, Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20150901
• Source Full Name:
  Analytical Chemistry
• End Date:
  20190831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:32410fac37572bf88587e02f3df78dfeb8f3fec67b4aa7928c42622c80868dd7cfe4f4d47094f2477e1ec1c532916acd573dd9e1c0e402cfa83cf439793ea0f0
• Download URL:
  https://stacks.cdc.gov/view/cdc/212260/cdc_212260_DS1.pdf
• File Type:
  [PDF - 4.11 MB ]