Circadian Rhythm of Circulating Levels of the Endocannabinoid 2-Arachidonoylglycerol

Details

• Personal Author:
  de Wit H ; Doncheck E ; Hanlon EC ; Hillard CJ ; Leproult R ; Stuhr KL ; Tasali E ; Van Cauter E
• Description:
  Context: The endocannabinoid (eCB) system is involved in the regulation of food intake and of peripheral metabolism. Although the cross talk between energy metabolism and the circadian system is well documented, little is known about a potential circadian modulation of human eCB activity. Objective: The objective of the study was to define the 24-hour profile of circulating levels of the most abundant endogenous ligand of the CB1 receptor, 2-arachidonoylglycerol (2-AG), in healthy young nonobese adults studied under controlled bedtime, dietary, and activity conditions. Methods: Fourteen subjects participated in this 4-day laboratory study with fixed light-dark cycles, standardized meals, and bedtimes. Sleep was recorded each night. On the third day, blood sampling at 15- to 30-minute intervals began at 9:30 pm and continued for 24 hours. Cortisol, leptin, and ghrelin were assayed on all samples, whereas the levels of 2-AG and its structural analog, 2-oleoylglycerol (2-OG), were measured at 60-minute intervals. Results: All participants exhibited a large circadian variation of 2-AG serum concentrations with a nadir around midsleep, coincident with the middle of the overnight fast. Levels of 2-AG increased continually across the morning, peaking in the early to midafternoon. Peak values represented, on average, a nearly 3-fold increase above nocturnal nadir levels. Concentrations of 2-OG followed a similar pattern, although with a shorter morning increase and lower amplitude. Conclusions: The findings demonstrate that activity of the eCB system is profoundly modulated by circadian rhythmicity and suggest that its impact on the regulation of food intake is suppressed during sleep and is maximal during early to midafternoon. [Description provided by NIOSH]
• Subjects:
  Circadian Rhythm Energy Metabolism Occupational Health Risk Assessment Risk Factors Safety Sleep Sleep Initiation And Maintenance Disorders
• Keywords:
  Biological Function Circadian Rhythms Hormone Activity Metabolic Effects Obesity Risk Factors Sleep Deprivation Sleep Disorders
• ISSN:
  0021-972X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5 ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  220-226
• Volume:
  100
• Issue:
  1
• NIOSHTIC Number:
  nn:20052727
• Citation:
  J Clin Endocrinol Metab 2015 Jan; 100(1):220-226
• Contact Point Address:
  Erin C. Hanlon, PhD, Department of Medicine, Section of Endocrinology, Sleep, Metabolism, and Health Center, University of Chicago, 5841 South Maryland Avenue, MC1027, Chicago, IL 60637
• Email:
  ehanlon@medicine.bsd.uchicago.edu
• Federal Fiscal Year:
  2015
• Performing Organization:
  University of Chicago
• Peer Reviewed:
  True
• Start Date:
  20090901
• Source Full Name:
  Journal of Clinical Endocrinology and Metabolism
• End Date:
  20150831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dd1a875ef07bb2ea410ab696d00649617b17f4dddf291e1ce214dc83efcf77c23453001b2884f01cb9e6bcb26f01e1dd4be74ea9160e38ce55504a3635abe110
• Download URL:
  https://stacks.cdc.gov/view/cdc/212207/cdc_212207_DS1.pdf
• File Type:
  [PDF - 148.16 KB ]